1k78: Difference between revisions

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New page: left|200px<br /> <applet load="1k78" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k78, resolution 2.25Å" /> '''Pax5(1-149)+Ets-1(3...
 
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[[Image:1k78.gif|left|200px]]<br />
<applet load="1k78" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1k78, resolution 2.25&Aring;" />
'''Pax5(1-149)+Ets-1(331-440)+DNA'''<br />


==Overview==
==Pax5(1-149)+Ets-1(331-440)+DNA==
Pax5 regulates the B cell-specific expression of the mb-1 gene together, with members of the Ets family of transcriptional activators. The Ets, proteins on their own bind poorly to the Pax5/Ets binding site, but can be, recruited to the site by cooperative interactions with Pax5. The structure, of the ETS domain of Ets-1 and the paired domain of Pax5 bound to DNA, reveals the molecular details of the selective recruitment of different, Ets proteins by Pax5. Comparison with structures of Ets-1 alone bound to, both high- and low-affinity DNA sites reveals that Pax5 alters the Ets-1, contacts with DNA. The ability of one protein to alter the DNA, sequence-specific contacts of another provides a general mechanism for, combinatorial regulation of transcription.
<StructureSection load='1k78' size='340' side='right'caption='[[1k78]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1k78]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K78 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K78 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k78 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k78 OCA], [https://pdbe.org/1k78 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k78 RCSB], [https://www.ebi.ac.uk/pdbsum/1k78 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k78 ProSAT]</span></td></tr>
</table>
== Disease ==
[https://www.uniprot.org/uniprot/PAX5_HUMAN PAX5_HUMAN] Note=A chromosomal aberration involving PAX5 is a cause of acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with ZNF521. Translocation t(9;3)(p13;p14.1) with FOXP1. Translocation t(9;12)(p13;p13) with ETV6.
== Function ==
[https://www.uniprot.org/uniprot/PAX5_HUMAN PAX5_HUMAN] May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k7/1k78_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k78 ConSurf].
<div style="clear:both"></div>


==Disease==
==See Also==
Known disease associated with this structure: Lymphoplasmacytoid lymphoma OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=167414 167414]]
*[[Ets1|Ets1]]
 
*[[Paired box protein|Paired box protein]]
==About this Structure==
__TOC__
1K78 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K78 OCA].
</StructureSection>
 
==Reference==
Structural studies of Ets-1/Pax5 complex formation on DNA., Garvie CW, Hagman J, Wolberger C, Mol Cell. 2001 Dec;8(6):1267-76. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11779502 11779502]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Protein complex]]
[[Category: Garvie CW]]
[[Category: Garvie, C.W.]]
[[Category: Hagman J]]
[[Category: Hagman, J.]]
[[Category: Wolberger C]]
[[Category: Wolberger, C.]]
[[Category: ets domain]]
[[Category: paired domain]]
[[Category: transcription factor]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:48:04 2007''

Latest revision as of 10:44, 7 February 2024

Pax5(1-149)+Ets-1(331-440)+DNAPax5(1-149)+Ets-1(331-440)+DNA

Structural highlights

1k78 is a 9 chain structure with sequence from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.25Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

PAX5_HUMAN Note=A chromosomal aberration involving PAX5 is a cause of acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with ZNF521. Translocation t(9;3)(p13;p14.1) with FOXP1. Translocation t(9;12)(p13;p13) with ETV6.

Function

PAX5_HUMAN May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1k78, resolution 2.25Å

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