1k66: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
New page: left|200px<br /><applet load="1k66" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k66, resolution 1.75Å" /> '''Crystal Structure of...
 
No edit summary
 
(14 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1k66.jpg|left|200px]]<br /><applet load="1k66" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1k66, resolution 1.75&Aring;" />
'''Crystal Structure of the Cyanobacterial Phytochrome Response Regulator, RcpB'''<br />


==Overview==
==Crystal Structure of the Cyanobacterial Phytochrome Response Regulator, RcpB==
The structures of two response regulators (RRs) from the cyanobacterium, Calothrix PCC7601, RcpA and RcpB, were solved to 1.9- and 1.75-A, resolution, respectively. RcpA was found in phosphorylated and RcpB in, nonphosphorylated form. Both RRs are members of phytochrome-associated, light-sensing two-component signal transduction pathways, based on, histidine kinase-mediated receptor autophosphorylation and phosphorelay to, a RR. Despite the overall folding similarity to CheY-type RRs, ((beta/alpha)(5)-motif), RcpA and RcpB form homodimers, irrespective of, their phosphorylation state, giving insight into a signal transduction, putatively different from that of other known RRs. Dimerization is, accomplished by a C-terminal extension of the RR polypeptide chain, and, the surface formed by H4, beta 5, and H5, which constitute a hydrophobic, contact area with distinct interactions between residues of either, subunit. Sequence alignments reveal that the identified dimerization motif, is archetypal for phytochrome-associated RRs, making them a novel subgroup, of CheY-type RRs. The protein structures of RcpA and RcpB are compared to, the recently presented protein structure of Rcp1 from Synechocystis.
<StructureSection load='1k66' size='340' side='right'caption='[[1k66]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1k66]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tolypothrix_sp._PCC_7601 Tolypothrix sp. PCC 7601]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K66 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K66 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k66 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k66 OCA], [https://pdbe.org/1k66 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k66 RCSB], [https://www.ebi.ac.uk/pdbsum/1k66 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k66 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q8RTM8_9CYAN Q8RTM8_9CYAN]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k6/1k66_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k66 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1K66 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Tolypothrix_sp._pcc_7601 Tolypothrix sp. pcc 7601] with BME as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K66 OCA].
*[[Response regulator 3D structure|Response regulator 3D structure]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structures of two cyanobacterial response regulators in apo- and phosphorylated form reveal a novel dimerization motif of phytochrome-associated response regulators., Benda C, Scheufler C, Tandeau de Marsac N, Gartner W, Biophys J. 2004 Jul;87(1):476-87. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15240481 15240481]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Tolypothrix sp. PCC 7601]]
[[Category: Tolypothrix sp. pcc 7601]]
[[Category: Benda C]]
[[Category: Benda, C.]]
[[Category: Gaertner W]]
[[Category: Gaertner, W.]]
[[Category: Scheufler C]]
[[Category: Marsac, N.Tandeau.de.]]
[[Category: Tandeau de Marsac N]]
[[Category: Scheufler, C.]]
[[Category: BME]]
[[Category: (beta/alpha)5]]
[[Category: apo-protein]]
[[Category: chey homologue]]
[[Category: homodimer]]
[[Category: response regulator]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:49:04 2007''

Latest revision as of 10:44, 7 February 2024

Crystal Structure of the Cyanobacterial Phytochrome Response Regulator, RcpBCrystal Structure of the Cyanobacterial Phytochrome Response Regulator, RcpB

Structural highlights

1k66 is a 2 chain structure with sequence from Tolypothrix sp. PCC 7601. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.75Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q8RTM8_9CYAN

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1k66, resolution 1.75Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=1k66&oldid=4045685"