1k4k: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
(11 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1k4k.jpg|left|200px]]


{{Structure
==Crystal structure of E. coli Nicotinic acid mononucleotide adenylyltransferase==
|PDB= 1k4k |SIZE=350|CAPTION= <scene name='initialview01'>1k4k</scene>, resolution 2.00&Aring;
<StructureSection load='1k4k' size='340' side='right'caption='[[1k4k]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=XE:XENON'>XE</scene>
<table><tr><td colspan='2'>[[1k4k]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K4K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K4K FirstGlance]. <br>
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nicotinate-nucleotide_adenylyltransferase Nicotinate-nucleotide adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.18 2.7.7.18] </span>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
|GENE= NADD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=XE:XENON'>XE</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k4k OCA], [https://pdbe.org/1k4k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k4k RCSB], [https://www.ebi.ac.uk/pdbsum/1k4k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k4k ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k4k OCA], [http://www.ebi.ac.uk/pdbsum/1k4k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1k4k RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/NADD_ECOLI NADD_ECOLI] Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD).
 
== Evolutionary Conservation ==
'''Crystal structure of E. coli Nicotinic acid mononucleotide adenylyltransferase'''
[[Image:Consurf_key_small.gif|200px|right]]
 
Check<jmol>
 
  <jmolCheckbox>
==Overview==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k4/1k4k_consurf.spt"</scriptWhenChecked>
Nicotinamide/Nicotinate mononucleotide (NMN/NaMN) adenylyltransferase is an indispensable enzyme in both de novo biosynthesis and salvage of NAD+ and NADP+. In prokaryotes, it is absolutely required for cell survival, thus representing an attractive target for the development of new broad-spectrum antibacteria inhibitors. The crystal structures of E. coli NaMN adenylyltransferase (NMNAT) and its complex with deamido-NAD (NaAD) revealed that ligand binding causes large conformational changes in several loop regions around the active site. The enzyme specifically recognizes the deamidated pyridine nucleotide through interactions between nicotinate carboxylate with several protein main chain amides and a positive helix dipole. Comparison of E. coli NMNAT with those from archaeal organisms revealed extensive differences in the active site architecture, enzyme-ligand interaction mode, and bound dinucleotide conformations. The bacterial NaMN adenylyltransferase structures described here provide a foundation for structure-based design of specific inhibitors that may have therapeutic potential.
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==About this Structure==
  </jmolCheckbox>
1K4K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K4K OCA].  
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k4k ConSurf].
 
<div style="clear:both"></div>
==Reference==
__TOC__
Crystal structures of E. coli nicotinate mononucleotide adenylyltransferase and its complex with deamido-NAD., Zhang H, Zhou T, Kurnasov O, Cheek S, Grishin NV, Osterman A, Structure. 2002 Jan;10(1):69-79. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11796112 11796112]
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Nicotinate-nucleotide adenylyltransferase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Cheek S]]
[[Category: Cheek, S.]]
[[Category: Grishin NV]]
[[Category: Grishin, N V.]]
[[Category: Kurnasov O]]
[[Category: Kurnasov, O.]]
[[Category: Osterman AL]]
[[Category: Osterman, A L.]]
[[Category: Zhang H]]
[[Category: Zhang, H.]]
[[Category: Zhou T]]
[[Category: Zhou, T.]]
[[Category: nucleotidyltransferase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:43:21 2008''

Latest revision as of 10:44, 7 February 2024

Crystal structure of E. coli Nicotinic acid mononucleotide adenylyltransferaseCrystal structure of E. coli Nicotinic acid mononucleotide adenylyltransferase

Structural highlights

1k4k is a 4 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NADD_ECOLI Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1k4k, resolution 2.00Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=1k4k&oldid=4045681"