1k0n: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1k0n.jpg|left|200px]]<br /><applet load="1k0n" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1k0n, resolution 1.80&Aring;" />
-'''Chloride Intracellular Channel 1 (CLIC1) complexed with glutathione'''<br />
-==Overview==
+==Chloride Intracellular Channel 1 (CLIC1) complexed with glutathione==
-CLIC1 (NCC27) is a member of the highly conserved class of chloride ion channels that exists in both soluble and integral membrane forms. Purified CLIC1 can integrate into synthetic lipid bilayers forming a chloride channel with similar properties to those observed in vivo. The structure of the soluble form of CLIC1 has been determined at 1.4-A resolution. The protein is monomeric and structurally homologous to the glutathione S-transferase superfamily, and it has a redox-active site resembling glutaredoxin. The structure of the complex of CLIC1 with glutathione shows that glutathione occupies the redox-active site, which is adjacent to an open, elongated slot lined by basic residues. Integration of CLIC1 into the membrane is likely to require a major structural rearrangement, probably of the N-domain (residues 1-90), with the putative transmembrane helix arising from residues in the vicinity of the redox-active site. The structure indicates that CLIC1 is likely to be controlled by redox-dependent processes.
+<StructureSection load='1k0n' size='340' side='right'caption='[[1k0n]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1k0n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K0N FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k0n OCA], [https://pdbe.org/1k0n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k0n RCSB], [https://www.ebi.ac.uk/pdbsum/1k0n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k0n ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CLIC1_HUMAN CLIC1_HUMAN] Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.<ref>PMID:9139710</ref> <ref>PMID:10834939</ref> <ref>PMID:11195932</ref> <ref>PMID:11940526</ref> <ref>PMID:11978800</ref> <ref>PMID:11551966</ref> <ref>PMID:14613939</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k0/1k0n_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k0n ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-K0N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=GTT:'>GTT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0N OCA].
+*[[Ion channels 3D structures|Ion channels 3D structures]]
+== References ==
-==Reference==
+<references/>
-Crystal structure of a soluble form of the intracellular chloride ion channel CLIC1 (NCC27) at 1.4-A resolution., Harrop SJ, DeMaere MZ, Fairlie WD, Reztsova T, Valenzuela SM, Mazzanti M, Tonini R, Qiu MR, Jankova L, Warton K, Bauskin AR, Wu WM, Pankhurst S, Campbell TJ, Breit SN, Curmi PM, J Biol Chem. 2001 Nov 30;276(48):44993-5000. Epub 2001 Sep 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11551966 11551966]
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Bauskin, A R.]]
+[[Category: Bauskin AR]]
-[[Category: Breit, S N.]]
+[[Category: Breit SN]]
-[[Category: Campbell, T J.]]
+[[Category: Campbell TJ]]
-[[Category: Curmi, P M.G.]]
+[[Category: Curmi PMG]]
-[[Category: DeMaere, M Z.]]
+[[Category: DeMaere MZ]]
-[[Category: Fairlie, W D.]]
+[[Category: Fairlie WD]]
-[[Category: Harrop, S J.]]
+[[Category: Harrop SJ]]
-[[Category: Jankova, L.]]
+[[Category: Jankova L]]
-[[Category: Mazzanti, M.]]
+[[Category: Mazzanti M]]
-[[Category: Pankhurst, S.]]
+[[Category: Pankhurst S]]
-[[Category: Qiu, M R.]]
+[[Category: Qiu MR]]
-[[Category: Reztsova, T.]]
+[[Category: Reztsova T]]
-[[Category: Tonini, R.]]
+[[Category: Tonini R]]
-[[Category: Valenzuela, S M.]]
+[[Category: Valenzuela SM]]
-[[Category: Warton, K.]]
+[[Category: Warton K]]
-[[Category: Wu, W M.]]
+[[Category: Wu WM]]
-[[Category: GTT]]
-[[Category: chloride ion channel]]
-[[Category: clic1]]
-[[Category: glutathione]]
-[[Category: glutathione-s-tranferase superfamily]]
-[[Category: ncc27]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:28:49 2008''