1k0m: Difference between revisions

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New page: left|200px<br /> <applet load="1k0m" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k0m, resolution 1.40Å" /> '''Crystal structure o...
 
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[[Image:1k0m.gif|left|200px]]<br />
<applet load="1k0m" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1k0m, resolution 1.40&Aring;" />
'''Crystal structure of a soluble monomeric form of CLIC1 at 1.4 angstroms'''<br />


==Overview==
==Crystal structure of a soluble monomeric form of CLIC1 at 1.4 angstroms==
CLIC1 (NCC27) is a member of the highly conserved class of chloride ion, channels that exists in both soluble and integral membrane forms. Purified, CLIC1 can integrate into synthetic lipid bilayers forming a chloride, channel with similar properties to those observed in vivo. The structure, of the soluble form of CLIC1 has been determined at 1.4-A resolution. The, protein is monomeric and structurally homologous to the glutathione, S-transferase superfamily, and it has a redox-active site resembling, glutaredoxin. The structure of the complex of CLIC1 with glutathione shows, that glutathione occupies the redox-active site, which is adjacent to an, open, elongated slot lined by basic residues. Integration of CLIC1 into, the membrane is likely to require a major structural rearrangement, probably of the N-domain (residues 1-90), with the putative transmembrane, helix arising from residues in the vicinity of the redox-active site. The, structure indicates that CLIC1 is likely to be controlled by, redox-dependent processes.
<StructureSection load='1k0m' size='340' side='right'caption='[[1k0m]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1k0m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K0M FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k0m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k0m OCA], [https://pdbe.org/1k0m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k0m RCSB], [https://www.ebi.ac.uk/pdbsum/1k0m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k0m ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CLIC1_HUMAN CLIC1_HUMAN] Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.<ref>PMID:9139710</ref> <ref>PMID:10834939</ref> <ref>PMID:11195932</ref> <ref>PMID:11940526</ref> <ref>PMID:11978800</ref> <ref>PMID:11551966</ref> <ref>PMID:14613939</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k0/1k0m_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k0m ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1K0M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1K0M OCA].
*[[Ion channels 3D structures|Ion channels 3D structures]]
 
== References ==
==Reference==
<references/>
Crystal structure of a soluble form of the intracellular chloride ion channel CLIC1 (NCC27) at 1.4-A resolution., Harrop SJ, DeMaere MZ, Fairlie WD, Reztsova T, Valenzuela SM, Mazzanti M, Tonini R, Qiu MR, Jankova L, Warton K, Bauskin AR, Wu WM, Pankhurst S, Campbell TJ, Breit SN, Curmi PM, J Biol Chem. 2001 Nov 30;276(48):44993-5000. Epub 2001 Sep 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11551966 11551966]
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bauskin, A.R.]]
[[Category: Bauskin AR]]
[[Category: Breit, S.N.]]
[[Category: Breit SN]]
[[Category: Campbell, T.J.]]
[[Category: Campbell TJ]]
[[Category: Curmi, P.M.G.]]
[[Category: Curmi PMG]]
[[Category: DeMaere, M.Z.]]
[[Category: DeMaere MZ]]
[[Category: Fairlie, W.D.]]
[[Category: Fairlie WD]]
[[Category: Harrop, S.J.]]
[[Category: Harrop SJ]]
[[Category: Jankova, L.]]
[[Category: Jankova L]]
[[Category: Mazzanti, M.]]
[[Category: Mazzanti M]]
[[Category: Pankhurst, S.]]
[[Category: Pankhurst S]]
[[Category: Qiu, M.R.]]
[[Category: Qiu MR]]
[[Category: Reztsova, T.]]
[[Category: Reztsova T]]
[[Category: Tonini, R.]]
[[Category: Tonini R]]
[[Category: Valenzuela, S.M.]]
[[Category: Valenzuela SM]]
[[Category: Warton, K.]]
[[Category: Warton K]]
[[Category: Wu, W.M.]]
[[Category: Wu WM]]
[[Category: chloride ion channel]]
[[Category: glutathione-s-tranferase superfamily]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:46:06 2007''

Latest revision as of 10:43, 7 February 2024

Crystal structure of a soluble monomeric form of CLIC1 at 1.4 angstromsCrystal structure of a soluble monomeric form of CLIC1 at 1.4 angstroms

Structural highlights

1k0m is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.4Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CLIC1_HUMAN Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.[1] [2] [3] [4] [5] [6] [7]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Valenzuela SM, Martin DK, Por SB, Robbins JM, Warton K, Bootcov MR, Schofield PR, Campbell TJ, Breit SN. Molecular cloning and expression of a chloride ion channel of cell nuclei. J Biol Chem. 1997 May 9;272(19):12575-82. PMID:9139710
  2. ↑ Tonini R, Ferroni A, Valenzuela SM, Warton K, Campbell TJ, Breit SN, Mazzanti M. Functional characterization of the NCC27 nuclear protein in stable transfected CHO-K1 cells. FASEB J. 2000 Jun;14(9):1171-8. PMID:10834939
  3. ↑ Valenzuela SM, Mazzanti M, Tonini R, Qiu MR, Warton K, Musgrove EA, Campbell TJ, Breit SN. The nuclear chloride ion channel NCC27 is involved in regulation of the cell cycle. J Physiol. 2000 Dec 15;529 Pt 3:541-52. PMID:11195932
  4. ↑ Tulk BM, Kapadia S, Edwards JC. CLIC1 inserts from the aqueous phase into phospholipid membranes, where it functions as an anion channel. Am J Physiol Cell Physiol. 2002 May;282(5):C1103-12. PMID:11940526 doi:10.1152/ajpcell.00402.2001
  5. ↑ Warton K, Tonini R, Fairlie WD, Matthews JM, Valenzuela SM, Qiu MR, Wu WM, Pankhurst S, Bauskin AR, Harrop SJ, Campbell TJ, Curmi PM, Breit SN, Mazzanti M. Recombinant CLIC1 (NCC27) assembles in lipid bilayers via a pH-dependent two-state process to form chloride ion channels with identical characteristics to those observed in Chinese hamster ovary cells expressing CLIC1. J Biol Chem. 2002 Jul 19;277(29):26003-11. Epub 2002 Apr 26. PMID:11978800 doi:http://dx.doi.org/10.1074/jbc.M203666200
  6. ↑ Harrop SJ, DeMaere MZ, Fairlie WD, Reztsova T, Valenzuela SM, Mazzanti M, Tonini R, Qiu MR, Jankova L, Warton K, Bauskin AR, Wu WM, Pankhurst S, Campbell TJ, Breit SN, Curmi PM. Crystal structure of a soluble form of the intracellular chloride ion channel CLIC1 (NCC27) at 1.4-A resolution. J Biol Chem. 2001 Nov 30;276(48):44993-5000. Epub 2001 Sep 10. PMID:11551966 doi:10.1074/jbc.M107804200
  7. ↑ Littler DR, Harrop SJ, Fairlie WD, Brown LJ, Pankhurst GJ, Pankhurst S, DeMaere MZ, Campbell TJ, Bauskin AR, Tonini R, Mazzanti M, Breit SN, Curmi PM. The intracellular chloride ion channel protein CLIC1 undergoes a redox-controlled structural transition. J Biol Chem. 2004 Mar 5;279(10):9298-305. Epub 2003 Nov 12. PMID:14613939 doi:http://dx.doi.org/10.1074/jbc.M308444200

1k0m, resolution 1.40Å

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