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[[Image:1jye.jpg|left|200px]]<br /><applet load="1jye" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1jye, resolution 1.70&Aring;" />
'''Structure of a Dimeric Lac Repressor with C-terminal Deletion and K84L Substitution'''<br />


==Overview==
==Structure of a Dimeric Lac Repressor with C-terminal Deletion and K84L Substitution==
A single amino acid substitution, K84L, in the Escherichia coli lac repressor produces a protein that has substantially increased stability compared to wild-type. However, despite the increased stability, this altered tetrameric repressor has a tenfold reduced affinity for operator and greatly decreased rate-constants of inducer binding as well as a reduced phenotypic response to inducer in vivo. To understand the dramatic increase in stability and altered functional properties, we have determined the X-ray crystal structures of a dimeric repressor with and without the K84L substitution at resolutions of 1.7 and 3.0 A, respectively. In the wild-type dimer, K84-11, Lys84 forms electrostatic interactions at the monomer-monomer interface and is partially exposed to solvent. In the K84L-11 substituted protein there is reorientation of the N-subdomains, which allows the leucine to become deeply buried at the monomer-monomer interface. This reorientation of the N-subdomains, in turn, results in an alteration of hydrogen bonding, ion pairing, and van der Waals interactions at the monomer-monomer interface. The lysine residue at position 84 appears to exert its key effects by destabilizing the "optimal" conformation of the repressor, effectively loosening the dimer interface and allowing the repressor to adopt the conformations necessary to function as a molecular switch.
<StructureSection load='1jye' size='340' side='right'caption='[[1jye]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1jye]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JYE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JYE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jye FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jye OCA], [https://pdbe.org/1jye PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jye RCSB], [https://www.ebi.ac.uk/pdbsum/1jye PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jye ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LACI_ECOLI LACI_ECOLI] Repressor of the lactose operon. Binds allolactose as an inducer.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jy/1jye_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jye ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1JYE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JYE OCA].
*[[Lac repressor|Lac repressor]]
 
__TOC__
==Reference==
</StructureSection>
Structure of a variant of lac repressor with increased thermostability and decreased affinity for operator., Bell CE, Barry J, Matthews KS, Lewis M, J Mol Biol. 2001 Oct 12;313(1):99-109. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11601849 11601849]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Barry, J.]]
[[Category: Barry J]]
[[Category: Bell, C E.]]
[[Category: Bell CE]]
[[Category: Lewis, M.]]
[[Category: Lewis M]]
[[Category: Matthews, K S.]]
[[Category: Matthews KS]]
[[Category: GOL]]
[[Category: gene regulation]]
[[Category: protein dna-binding]]
[[Category: protein stability]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:28:12 2008''

Latest revision as of 10:42, 7 February 2024

Structure of a Dimeric Lac Repressor with C-terminal Deletion and K84L SubstitutionStructure of a Dimeric Lac Repressor with C-terminal Deletion and K84L Substitution

Structural highlights

1jye is a 1 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.7Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LACI_ECOLI Repressor of the lactose operon. Binds allolactose as an inducer.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1jye, resolution 1.70Å

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