1jwn: Difference between revisions

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New page: left|200px<br /> <applet load="1jwn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jwn, resolution 2.10Å" /> '''Crystal Structure o...
 
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[[Image:1jwn.gif|left|200px]]<br />
<applet load="1jwn" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1jwn, resolution 2.10&Aring;" />
'''Crystal Structure of Scapharca inaequivalvis HbI, I114F Mutant Ligated to Carbon Monoxide.'''<br />


==Overview==
==Crystal Structure of Scapharca inaequivalvis HbI, I114F Mutant Ligated to Carbon Monoxide.==
Cooperative ligand binding in the dimeric hemoglobin from the blood clam, Scapharca inaequivalvis results primarily from tertiary, rather than, quaternary, structural changes. Ligand binding is coupled with, conformational changes of key residues, including Phe 97, which is, extruded from the proximal heme pocket, and the heme group, which moves, deeper into the heme pocket. We have tested the role of the heme movement, in cooperative function by mutating Ile 114, at the base of the heme, pocket. Replacement of this residue with a Met did not disturb the, hemoglobin structure or significantly alter equilibrium ligand binding, properties. In contrast, substitution with a Phe at position 114 inhibits, the ligand-linked movement of the heme group, and substantially reduces, oxygen affinity and cooperativity. As the extent of heme movement to the, normal position of the ligated state is diminished, Phe 97 is inhibited, from its movement into the interface upon ligand binding. These results, indicate a tight coupling between these two key cooperative transitions, and suggest that the heme movement may be an obligatory trigger for, expulsion of Phe 97 from the heme pocket.
<StructureSection load='1jwn' size='340' side='right'caption='[[1jwn]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1jwn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Anadara_inaequivalvis Anadara inaequivalvis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JWN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jwn OCA], [https://pdbe.org/1jwn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jwn RCSB], [https://www.ebi.ac.uk/pdbsum/1jwn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jwn ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GLB1_ANAIN GLB1_ANAIN]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jw/1jwn_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jwn ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1JWN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Scapharca_inaequivalvis Scapharca inaequivalvis] with HEM and CMO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JWN OCA].
*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Restricting the ligand-linked heme movement in Scapharca dimeric hemoglobin reveals tight coupling between distal and proximal contributions to cooperativity., Knapp JE, Gibson QH, Cushing L, Royer WE Jr, Biochemistry. 2001 Dec 11;40(49):14795-805. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11732898 11732898]
[[Category: Anadara inaequivalvis]]
[[Category: Scapharca inaequivalvis]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Cushing L]]
[[Category: Cushing, L.]]
[[Category: Gibson QH]]
[[Category: Gibson, Q.H.]]
[[Category: Knapp JE]]
[[Category: Jr., W.E.Royer.]]
[[Category: Royer Jr WE]]
[[Category: Knapp, J.E.]]
[[Category: CMO]]
[[Category: HEM]]
[[Category: allostery]]
[[Category: cooperativity]]
[[Category: heme protein]]
[[Category: invertebrate hemoglobin]]
[[Category: oxygen-binding protein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov  8 13:10:31 2007''

Latest revision as of 10:42, 7 February 2024

Crystal Structure of Scapharca inaequivalvis HbI, I114F Mutant Ligated to Carbon Monoxide.Crystal Structure of Scapharca inaequivalvis HbI, I114F Mutant Ligated to Carbon Monoxide.

Structural highlights

1jwn is a 4 chain structure with sequence from Anadara inaequivalvis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.1Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GLB1_ANAIN

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1jwn, resolution 2.10Å

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