1jt0: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1jt0.gif|left|200px]]<br /><applet load="1jt0" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1jt0, resolution 2.90&Aring;" />
-'''Crystal structure of a cooperative QacR-DNA complex'''<br />
-==Overview==
+==Crystal structure of a cooperative QacR-DNA complex==
-The Staphylococcus aureus multidrug-binding protein QacR represses, transcription of the qacA multidrug transporter gene and is induced by, multiple structurally dissimilar drugs. QacR is a member of the TetR/CamR, family of transcriptional regulators, which share highly homologous, N-terminal DNA-binding domains connected to seemingly non-homologous, ligand-binding domains. Unlike other TetR members, which bind, approximately 15 bp operators, QacR recognizes an unusually long 28 bp, operator, IR1, which it appears to bind cooperatively. To elucidate the, DNA-binding mechanism of QacR, we determined the 2.90 A resolution crystal, structure of a QacR-IR1 complex. Strikingly, our data reveal that the DNA, recognition mode of QacR is distinct from TetR and involves the binding of, a pair of QacR dimers. In this unique binding mode, recognition at each, IR1 half-site is mediated by a complement of DNA contacts made by two, helix-turn-helix motifs. The inferred cooperativity does not arise from, cross-dimer protein-protein contacts, but from the global undertwisting, and major groove widening elicited by the binding of two QacR dimers.
+<StructureSection load='1jt0' size='340' side='right'caption='[[1jt0]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1jt0]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JT0 FirstGlance]. <br>
-JT0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JT0 OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jt0 OCA], [https://pdbe.org/1jt0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jt0 RCSB], [https://www.ebi.ac.uk/pdbsum/1jt0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jt0 ProSAT]</span></td></tr>
-Structural basis for cooperative DNA binding by two dimers of the multidrug-binding protein QacR., Schumacher MA, Miller MC, Grkovic S, Brown MH, Skurray RA, Brennan RG, EMBO J. 2002 Mar 1;21(5):1210-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11867549 11867549]
+</table>
-[[Category: Single protein]]
+== Function ==
+[https://www.uniprot.org/uniprot/QACR_STAAU QACR_STAAU] Transcriptional repressor of qacA. Binds to IR1, an unusually long 28 bp operator, which is located downstream from the qacA promoter and overlaps its transcription start site. QacR is induced from its IR1 site by binding to one of many structurally dissimilar cationic lipophilic compounds, which are also substrates of QacA.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/1jt0_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jt0 ConSurf].
+<div style="clear:both"></div>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Staphylococcus aureus]]
-[[Category: Brennan, R.G.]]
+[[Category: Brennan RG]]
-[[Category: Brown, M.H.]]
+[[Category: Brown MH]]
-[[Category: Grkovic, S.]]
+[[Category: Grkovic S]]
-[[Category: Miller, M.C.]]
+[[Category: Miller MC]]
-[[Category: Schumacher, M.A.]]
+[[Category: Schumacher MA]]
-[[Category: Skurray, R.A.]]
+[[Category: Skurray RA]]
-[[Category: SO4]]
-[[Category: cooperative dna binding]]
-[[Category: dimer of dimers]]
-[[Category: multidrug binding protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:33:13 2007''