1jl7: Difference between revisions

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-[[Image:1jl7.jpg|left|200px]]<br /><applet load="1jl7" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1jl7, resolution 1.4&Aring;" />
-'''Crystal Structure Of CN-Ligated Component III Glycera Dibranchiata Monomeric Hemoglobin'''<br />
-==Overview==
+==Crystal Structure Of CN-Ligated Component III Glycera Dibranchiata Monomeric Hemoglobin==
-Erythrocytes of the marine annelid, Glycera dibranchiata, contain a mixture of monomeric and polymeric hemoglobins. There are three major monomer hemoglobin components, II, III, IV (also called GMH2, 3, and 4), that have been highly purified and well characterized. We have now crystallized GMH3 and GMH4 and determined their structures to 1.4-1.8 A resolution. The structures were determined for these two monomer hemoglobins in the oxidized (Fe3+, ferric, or met-) forms in both the unligated and cyanide-ligated states. This work differs from two published, refined structures of a Glycera dibranchiata monomer hemoglobin, which has a sequence that is substantially different from any bona fide major monomer hemoglobins (GMH2, 3, or 4). The high-resolution crystal structures (presented here) and the previous NMR structure of CO-ligated GMH4, provide a basis for interpreting structure/function details of the monomer hemoglobins. These details include: (1) the strong correlation between temperature factor and NMR dynamics for respective protein forms; (2) the unique nature of the HisE7Leu primary sequence substitutions in GMH3 and GMH4 and their impact on cyanide ion binding kinetics; (3) the LeuB10Phe difference between GMH3 and GMH4 and its impact on ligand binding; and (4) elucidation of changes in the structural details of the distal and proximal heme pockets upon cyanide binding.
+<StructureSection load='1jl7' size='340' side='right'caption='[[1jl7]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1jl7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Glycera_dibranchiata Glycera dibranchiata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JL7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JL7 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYN:CYANIDE+ION'>CYN</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jl7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jl7 OCA], [https://pdbe.org/1jl7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jl7 RCSB], [https://www.ebi.ac.uk/pdbsum/1jl7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jl7 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/GLB1_GLYDI GLB1_GLYDI]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jl/1jl7_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jl7 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-JL7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Glycera_dibranchiata Glycera dibranchiata] with <scene name='pdbligand=CYN:'>CYN</scene> and <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JL7 OCA].
+*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Crystal structures of unligated and CN-ligated Glycera dibranchiata monomer ferric hemoglobin components III and IV., Park HJ, Yang C, Treff N, Satterlee JD, Kang C, Proteins. 2002 Oct 1;49(1):49-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12211015 12211015]
 [[Category: Glycera dibranchiata]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Kang, C.]]
+[[Category: Kang C]]
-[[Category: Park, H J.]]
+[[Category: Park HJ]]
-[[Category: Satterlee, J D.]]
+[[Category: Satterlee JD]]
-[[Category: Treff, N.]]
+[[Category: Treff N]]
-[[Category: Yang, C.]]
+[[Category: Yang C]]
-[[Category: CYN]]
-[[Category: HEM]]
-[[Category: glycera]]
-[[Category: monomer hemoglobin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:23:55 2008''