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[[Image:1jku.gif|left|200px]]<br /><applet load="1jku" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1jku, resolution 1.84&Aring;" />
'''Crystal Structure of Manganese Catalase from Lactobacillus plantarum'''<br />


==Overview==
==Crystal Structure of Manganese Catalase from Lactobacillus plantarum==
BACKGROUND: Catalases are important antioxidant metalloenzymes that catalyze disproportionation of hydrogen peroxide, forming dioxygen and water. Two families of catalases are known, one having a heme cofactor, and the other, a structurally distinct family containing nonheme manganese. We have solved the structure of the mesophilic manganese catalase from Lactobacillus plantarum and its azide-inhibited complex. RESULTS: The crystal structure of the native enzyme has been solved at 1.8 A resolution by molecular replacement, and the azide complex of the native protein has been solved at 1.4 A resolution. The hexameric structure of the holoenzyme is stabilized by extensive intersubunit contacts, including a beta zipper and a structural calcium ion crosslinking neighboring subunits. Each subunit contains a dimanganese active site, accessed by a single substrate channel lined by charged residues. The manganese ions are linked by a mu1,3-bridging glutamate carboxylate and two mu-bridging solvent oxygens that electronically couple the metal centers. The active site region includes two residues (Arg147 and Glu178) that appear to be unique to the Lactobacillus plantarum catalase. CONCLUSIONS: A comparison of L. plantarum and T. thermophilus catalase structures reveals the existence of two distinct structural classes, differing in monomer design and the organization of their active sites, within the manganese catalase family. These differences have important implications for catalysis and may reflect distinct biological functions for the two enzymes, with the L. plantarum enzyme serving as a catalase, while the T. thermophilus enzyme may function as a catalase/peroxidase.
<StructureSection load='1jku' size='340' side='right'caption='[[1jku]], [[Resolution|resolution]] 1.84&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1jku]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactiplantibacillus_plantarum Lactiplantibacillus plantarum]. The September 2004 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Catalase''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2004_9 10.2210/rcsb_pdb/mom_2004_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JKU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JKU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.84&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN3:MANGANESE+(III)+ION'>MN3</scene>, <scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jku FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jku OCA], [https://pdbe.org/1jku PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jku RCSB], [https://www.ebi.ac.uk/pdbsum/1jku PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jku ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MCAT_LACPN MCAT_LACPN]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jk/1jku_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jku ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1JKU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lactobacillus_plantarum Lactobacillus plantarum] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=OH:'>OH</scene> and <scene name='pdbligand=MN3:'>MN3</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1JKU with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb57_1.html Catalase]]. Active as [http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JKU OCA].
*[[Catalase|Catalase]]
 
*[[Catalase 3D structures|Catalase 3D structures]]
==Reference==
__TOC__
Crystal structure of manganese catalase from Lactobacillus plantarum., Barynin VV, Whittaker MM, Antonyuk SV, Lamzin VS, Harrison PM, Artymiuk PJ, Whittaker JW, Structure. 2001 Aug;9(8):725-38. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11587647 11587647]
</StructureSection>
[[Category: Catalase]]
[[Category: Catalase]]
[[Category: Lactobacillus plantarum]]
[[Category: Lactiplantibacillus plantarum]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Antonyuk, S V.]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Artymiuk, P J.]]
[[Category: Antonyuk SV]]
[[Category: Barynin, V V.]]
[[Category: Artymiuk PJ]]
[[Category: Harrison, P M.]]
[[Category: Barynin VV]]
[[Category: Lamzin, V S.]]
[[Category: Harrison PM]]
[[Category: Whittaker, J W.]]
[[Category: Lamzin VS]]
[[Category: Whittaker, M M.]]
[[Category: Whittaker JW]]
[[Category: CA]]
[[Category: Whittaker MM]]
[[Category: MN3]]
[[Category: OH]]
[[Category: catalase dimanganese]]
[[Category: hexamer]]
[[Category: metalloenzyme]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:23:46 2008''

Latest revision as of 10:40, 7 February 2024

Crystal Structure of Manganese Catalase from Lactobacillus plantarumCrystal Structure of Manganese Catalase from Lactobacillus plantarum

Structural highlights

1jku is a 6 chain structure with sequence from Lactiplantibacillus plantarum. The September 2004 RCSB PDB Molecule of the Month feature on Catalase by David S. Goodsell is 10.2210/rcsb_pdb/mom_2004_9. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.84Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MCAT_LACPN

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1jku, resolution 1.84Å

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