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[[Image:1jhd.jpg|left|200px]]<br /><applet load="1jhd" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1jhd, resolution 1.70&Aring;" />
'''Crystal Structure of Bacterial ATP Sulfurylase from the Riftia pachyptila Symbiont'''<br />


==Overview==
==Crystal Structure of Bacterial ATP Sulfurylase from the Riftia pachyptila Symbiont==
In sulfur chemolithotrophic bacteria, the enzyme ATP sulfurylase functions to produce ATP and inorganic sulfate from APS and inorganic pyrophosphate, which is the final step in the biological oxidation of hydrogen sulfide to sulfate. The giant tubeworm, Riftia pachyptila, which lives near hydrothermal vents on the ocean floor, harbors a sulfur chemolithotroph as an endosymbiont in its trophosome tissue. This yet-to-be-named bacterium was found to contain high levels of ATP sulfurylase that may provide a substantial fraction of the organisms ATP. We present here, the crystal structure of ATP sulfurylase from this bacterium at 1.7 A resolution. As predicted from sequence homology, the enzyme folds into distinct N-terminal and catalytic domains, but lacks the APS kinase-like C-terminal domain that is present in fungal ATP sulfurylase. The enzyme crystallizes as a dimer with one subunit in the crystallographic asymmetric unit. Many buried solvent molecules mediate subunit contacts at the interface. Despite the high concentration of sulfate needed for crystallization, no ordered sulfate was observed in the sulfate-binding pocket. The structure reveals a mobile loop positioned over the active site. This loop is in a "closed" or "down" position in the reported crystal structures of fungal ATP sulfurylases, which contained bound substrates, but it is in an "open" or "up" position in the ligand-free Riftia symbiont enzyme. Thus, closure of the loop correlates with occupancy of the active site, although the loop itself does not interact directly with bound ligands. Rather, it appears to assist in the orientation of residues that do interact with active-site ligands. Amino acid differences between the mobile loops of the enzymes from sulfate assimilators and sulfur chemolithotrophs may account for the significant kinetic differences between the two classes of ATP sulfurylase.
<StructureSection load='1jhd' size='340' side='right'caption='[[1jhd]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1jhd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfur-oxidizing_endosymbiont_of_Riftia_pachyptila Sulfur-oxidizing endosymbiont of Riftia pachyptila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JHD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JHD FirstGlance]. <br>
1JHD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=BR:'>BR</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Sulfate_adenylyltransferase Sulfate adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.4 2.7.7.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JHD OCA].
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jhd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jhd OCA], [https://pdbe.org/1jhd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jhd RCSB], [https://www.ebi.ac.uk/pdbsum/1jhd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jhd ProSAT]</span></td></tr>
Crystal structure of ATP sulfurylase from the bacterial symbiont of the hydrothermal vent tubeworm Riftia pachyptila., Beynon JD, MacRae IJ, Huston SL, Nelson DC, Segel IH, Fisher AJ, Biochemistry. 2001 Dec 4;40(48):14509-17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11724564 11724564]
</table>
[[Category: Bacteria]]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/SAT_RIFPS SAT_RIFPS]
[[Category: Sulfate adenylyltransferase]]
== Evolutionary Conservation ==
[[Category: Beynon, J D.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Fisher, A J.]]
Check<jmol>
[[Category: Huston, S L.]]
  <jmolCheckbox>
[[Category: MacRae, I J.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jh/1jhd_consurf.spt"</scriptWhenChecked>
[[Category: Nelson, D C.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: Segel, I H.]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: BR]]
  </jmolCheckbox>
[[Category: SO4]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jhd ConSurf].
[[Category: adenylyl transferase]]
<div style="clear:both"></div>
[[Category: aps]]
__TOC__
[[Category: bromide]]
</StructureSection>
[[Category: chemoautotroph]]
[[Category: Large Structures]]
[[Category: sulfurylase]]
[[Category: Sulfur-oxidizing endosymbiont of Riftia pachyptila]]
 
[[Category: Beynon JD]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:22:37 2008''
[[Category: Fisher AJ]]
[[Category: Huston SL]]
[[Category: MacRae IJ]]
[[Category: Nelson DC]]
[[Category: Segel IH]]

Latest revision as of 10:39, 7 February 2024

Crystal Structure of Bacterial ATP Sulfurylase from the Riftia pachyptila SymbiontCrystal Structure of Bacterial ATP Sulfurylase from the Riftia pachyptila Symbiont

Structural highlights

1jhd is a 1 chain structure with sequence from Sulfur-oxidizing endosymbiont of Riftia pachyptila. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.7Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SAT_RIFPS

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1jhd, resolution 1.70Å

Drag the structure with the mouse to rotate

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OCA
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