1j90: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1j90.jpg|left|200px]]<br /><applet load="1j90" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1j90, resolution 2.56&Aring;" />
-'''Crystal Structure of Drosophila Deoxyribonucleoside Kinase'''<br />
-==Overview==
+==Crystal Structure of Drosophila Deoxyribonucleoside Kinase==
-Deoxyribonucleoside kinases phosphorylate deoxyribonucleosides and, activate a number of medically important nucleoside analogs. Here we, report the structure of the Drosophila deoxyribonucleoside kinase with, deoxycytidine bound at the nucleoside binding site and that of the human, deoxyguanosine kinase with ATP at the nucleoside substrate binding site., Compared to the human kinase, the Drosophila kinase has a wider substrate, cleft, which may be responsible for the broad substrate specificity of, this enzyme. The human deoxyguanosine kinase is highly specific for purine, substrates; this is apparently due to the presence of Arg 118, which, provides favorable hydrogen bonding interactions with the substrate. The, two new structures provide an explanation for the substrate specificity of, cellular deoxyribonucleoside kinases.
+<StructureSection load='1j90' size='340' side='right'caption='[[1j90]], [[Resolution|resolution]] 2.56&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1j90]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J90 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J90 FirstGlance]. <br>
-J90 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with SO4 and DCZ as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1J90 OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.56&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DCZ:2-DEOXYCYTIDINE'>DCZ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j90 OCA], [https://pdbe.org/1j90 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j90 RCSB], [https://www.ebi.ac.uk/pdbsum/1j90 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j90 ProSAT]</span></td></tr>
-Structural basis for substrate specificities of cellular deoxyribonucleoside kinases., Johansson K, Ramaswamy S, Ljungcrantz C, Knecht W, Piskur J, Munch-Petersen B, Eriksson S, Eklund H, Nat Struct Biol. 2001 Jul;8(7):616-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11427893 11427893]
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/DNK_DROME DNK_DROME] Deoxyribonucleoside kinase that has a broad specificity phosphorylating thymidine, deoxyadenosine, deoxycytidine and deoxyguanosine. Specificity is higher for pyrimidine nucleosides. Several anti-viral and anti-cancer nucleoside analogs are also efficiently phosphorylated.<ref>PMID:10446143</ref> <ref>PMID:10692477</ref> <ref>PMID:16008571</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j9/1j90_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j90 ConSurf].
+<div style="clear:both"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Drosophila melanogaster]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Eklund, H.]]
+[[Category: Eklund H]]
-[[Category: Eriksson, S.]]
+[[Category: Eriksson S]]
-[[Category: Johansson, K.]]
+[[Category: Johansson K]]
-[[Category: Knecht, W.]]
+[[Category: Knecht W]]
-[[Category: Ljungkrantz, C.]]
+[[Category: Ljungkrantz C]]
-[[Category: Munch-Petersen, B.]]
+[[Category: Munch-Petersen B]]
-[[Category: Piskur, J.]]
+[[Category: Piskur J]]
-[[Category: Ramaswamy, S.]]
+[[Category: Ramaswamy S]]
-[[Category: DCZ]]
-[[Category: SO4]]
-[[Category: protein-deoxynucleoside complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:01:00 2007''