1j8m: Difference between revisions

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New page: left|200px<br /><applet load="1j8m" size="450" color="white" frame="true" align="right" spinBox="true" caption="1j8m, resolution 2.00Å" /> '''Signal Recognition P...
 
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[[Image:1j8m.jpg|left|200px]]<br /><applet load="1j8m" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1j8m, resolution 2.00&Aring;" />
'''Signal Recognition Particle conserved GTPase domain from A. ambivalens'''<br />


==Overview==
==Signal Recognition Particle conserved GTPase domain from A. ambivalens==
BACKGROUND: Protein targeting to the endoplasmic reticulum in eukaryotes, and to the cell membrane in prokaryotes is mediated by the signal, recognition particle (SRP) and its receptor (SR). Both contain conserved, GTPase domains in the signal-peptide-binding proteins (SRP54 and Ffh) and, the SR proteins (SRalpha and FtsY). These GTPases are involved in the, regulation of protein targeting. Most studies so far have focussed on the, SRP machinery of mammals and bacteria, leaving the SRP system of archaea, less well understood. RESULTS: We report the crystal structure of the, conserved GTPase (NG-Ffh) from the thermophilic archaeon Acidianus, ambivalens at 2.0 A resolution and of the Thr112--&gt;Ala mutant, which is, inactive in GTP hydrolysis. This is the first structure of an SRP, component from an archaeon and allows for a detailed comparison with, related structures from Escherichia coli and thermophilic bacteria. In, particular, differences in the conserved consensus regions for nucleotide, binding and the subdomain interfaces are observed, which provide, information about the regulation of the GTPase. These interactions allow, us to propose a common signalling mechanism for the SRP-SR system., CONCLUSIONS: The overall structure of SRP-GTPases is well conserved, between bacteria and archaea, which indicates strong similarities in the, regulation of the SRP-targeting pathway. Surprisingly, structure, comparisons identified a homodimeric ATP-binding protein as the closest, relative. A heterodimer model for the SRP-SR interaction is presented.
<StructureSection load='1j8m' size='340' side='right'caption='[[1j8m]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1j8m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acidianus_ambivalens Acidianus ambivalens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J8M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J8M FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j8m OCA], [https://pdbe.org/1j8m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j8m RCSB], [https://www.ebi.ac.uk/pdbsum/1j8m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j8m ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SRP54_ACIAM SRP54_ACIAM] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j8/1j8m_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j8m ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1J8M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Acidianus_ambivalens Acidianus ambivalens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1J8M OCA].
*[[Signal recognition particle 3D structures|Signal recognition particle 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The crystal structure of the conserved GTPase of SRP54 from the archaeon Acidianus ambivalens and its comparison with related structures suggests a model for the SRP-SRP receptor complex., Montoya G, Kaat K, Moll R, Schafer G, Sinning I, Structure. 2000 May 15;8(5):515-25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10801496 10801496]
[[Category: Acidianus ambivalens]]
[[Category: Acidianus ambivalens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Kaat, K.te.]]
[[Category: Moll R]]
[[Category: Moll, R.]]
[[Category: Montoya G]]
[[Category: Montoya, G.]]
[[Category: Schafer G]]
[[Category: Schafer, G.]]
[[Category: Sinning I]]
[[Category: Sinning, I.]]
[[Category: Te Kaat K]]
[[Category: signaling protein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:00:45 2007''

Latest revision as of 10:37, 7 February 2024

Signal Recognition Particle conserved GTPase domain from A. ambivalensSignal Recognition Particle conserved GTPase domain from A. ambivalens

Structural highlights

1j8m is a 1 chain structure with sequence from Acidianus ambivalens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SRP54_ACIAM Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1j8m, resolution 2.00Å

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