1j7n: Difference between revisions

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-[[Image:1j7n.gif|left|200px]]
-<!--
+==Anthrax Toxin Lethal factor==
-The line below this paragraph, containing "STRUCTURE_1j7n", creates the "Structure Box" on the page.
+<StructureSection load='1j7n' size='340' side='right'caption='[[1j7n]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1j7n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J7N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J7N FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-{{STRUCTURE_1j7n|  PDB=1j7n  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j7n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j7n OCA], [https://pdbe.org/1j7n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j7n RCSB], [https://www.ebi.ac.uk/pdbsum/1j7n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j7n ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/LEF_BACAN LEF_BACAN] One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. LF is the lethal factor that, when associated with PA, causes death. LF is not toxic by itself. It is a protease that cleaves the N-terminal of most dual specificity mitogen-activated protein kinase kinases (MAPKKs or MAP2Ks) (except for MAP2K5). Cleavage invariably occurs within the N-terminal proline-rich region preceding the kinase domain, thus disrupting a sequence involved in directing specific protein-protein interactions necessary for the assembly of signaling complexes. There may be other cytosolic targets of LF involved in cytotoxicity. The proteasome may mediate a toxic process initiated by LF in the cell cytosol involving degradation of unidentified molecules that are essential for macrophage homeostasis. This is an early step in LeTx intoxication, but it is downstream of the cleavage by LF of MEK1 or other putative substrates.<ref>PMID:9563949</ref> <ref>PMID:9703991</ref> <ref>PMID:10475971</ref> <ref>PMID:11104681</ref> <ref>PMID:10338520</ref>
-'''Anthrax Toxin Lethal factor'''
+==See Also==
+*[[Anthrax Lethal Factor|Anthrax Lethal Factor]]
+*[[Anthrax lethal factor 3D structures|Anthrax lethal factor 3D structures]]
-==Overview==
+== References ==
-Lethal factor (LF) is a protein (relative molecular mass 90,000) that is critical in the pathogenesis of anthrax. It is a highly specific protease that cleaves members of the mitogen-activated protein kinase kinase (MAPKK) family near to their amino termini, leading to the inhibition of one or more signalling pathways. Here we describe the crystal structure of LF and its complex with the N terminus of MAPKK-2. LF comprises four domains: domain I binds the membrane-translocating component of anthrax toxin, the protective antigen (PA); domains II, III and IV together create a long deep groove that holds the 16-residue N-terminal tail of MAPKK-2 before cleavage. Domain II resembles the ADP-ribosylating toxin from Bacillus cereus, but the active site has been mutated and recruited to augment substrate recognition. Domain III is inserted into domain II, and seems to have arisen from a repeated duplication of a structural element of domain II. Domain IV is distantly related to the zinc metalloprotease family, and contains the catalytic centre; it also resembles domain I. The structure thus reveals a protein that has evolved through a process of gene duplication, mutation and fusion, into an enzyme with high and unusual specificity.
+<references/>
+__TOC__
-==About this Structure==
+</StructureSection>
-J7N is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J7N OCA].
-==Reference==
-Crystal structure of the anthrax lethal factor., Pannifer AD, Wong TY, Schwarzenbacher R, Renatus M, Petosa C, Bienkowska J, Lacy DB, Collier RJ, Park S, Leppla SH, Hanna P, Liddington RC, Nature. 2001 Nov 8;414(6860):229-33. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11700563 11700563]
 [[Category: Bacillus anthracis]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Bienkowska, J.]]
+[[Category: Bienkowska J]]
-[[Category: Collier, R J.]]
+[[Category: Collier RJ]]
-[[Category: Hanna, P.]]
+[[Category: Hanna P]]
-[[Category: Lacy, D B.]]
+[[Category: Lacy DB]]
-[[Category: Leppla, S H.]]
+[[Category: Leppla SH]]
-[[Category: Liddington, R C.]]
+[[Category: Liddington RC]]
-[[Category: Pannifer, A D.]]
+[[Category: Pannifer AD]]
-[[Category: Park, S.]]
+[[Category: Park S]]
-[[Category: Petosa, C.]]
+[[Category: Petosa C]]
-[[Category: Renatus, M.]]
+[[Category: Renatus M]]
-[[Category: Schwarzenbacher, R.]]
+[[Category: Schwarzenbacher R]]
-[[Category: Wong, T Y.]]
+[[Category: Wong TY]]
-[[Category: Anthrax]]
-[[Category: Lethal factor]]
-[[Category: Lethal toxin]]
-[[Category: Mapkk]]
-[[Category: Mek]]
-[[Category: Zinc metalloprotease]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 20:53:10 2008''