1ii9: Difference between revisions

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New page: left|200px<br /><applet load="1ii9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ii9, resolution 2.60Å" /> '''CRYSTAL STRUCTURE OF...
 
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[[Image:1ii9.gif|left|200px]]<br /><applet load="1ii9" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ii9, resolution 2.60&Aring;" />
'''CRYSTAL STRUCTURE OF THE ESCHERICHIA COLI ARSENITE-TRANSLOCATING ATPASE IN COMPLEX WITH AMP-PNP'''<br />


==Overview==
==CRYSTAL STRUCTURE OF THE ESCHERICHIA COLI ARSENITE-TRANSLOCATING ATPASE IN COMPLEX WITH AMP-PNP==
Structures of ArsA with ATP, AMP-PNP, or ADP.AlF(3) bound at the A2, nucleotide binding site were determined. Binding of different nucleotides, modifies the coordination sphere of Mg(2+). In particular, the changes, elicited by ADP.AlF(3) provide insights into the mechanism of ATP, hydrolysis. In-line attack by water onto the gamma-phosphate of ATP would, be followed first by formation of a trigonal intermediate and then by, breaking of the scissile bond between the beta- and gamma-phosphates., Motions of amino acid side chains at the A2 nucleotide binding site during, ATP binding and hydrolysis propagate at a distance, producing, conformational changes in four different regions of the protein, corresponding to helices H4-H5, helices H9-H10, helices H13-H15, and to, the S1-H2-S2 region. These elements are extensions of, respectively, the, Switch I and Switch II regions, the A-loop (a small loop near the, nucleotide adenine moiety), and the P-loop. Based on the observed, conformational changes, it is proposed that ArsA functions as a, reciprocating engine that hydrolyzes 2 mol of ATP per each cycle of ion, translocation across the membrane.
<StructureSection load='1ii9' size='340' side='right'caption='[[1ii9]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ii9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1II9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1II9 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TAS:TRIHYDROXYARSENITE(III)'>TAS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ii9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ii9 OCA], [https://pdbe.org/1ii9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ii9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ii9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ii9 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ARSA1_ECOLX ARSA1_ECOLX] Anion-transporting ATPase. Catalyzes the extrusion of the oxyanions arsenite, antimonite and arsenate. Maintenance of a low intracellular concentration of oxyanion produces resistance to the toxic agents.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ii/1ii9_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ii9 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1II9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG, CD, CL, ADP, ANP and TAS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Arsenite-transporting_ATPase Arsenite-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.16 3.6.3.16] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1II9 OCA].
*[[ATPase 3D structures|ATPase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Conformational changes in four regions of the Escherichia coli ArsA ATPase link ATP hydrolysis to ion translocation., Zhou T, Radaev S, Rosen BP, Gatti DL, J Biol Chem. 2001 Aug 10;276(32):30414-22. Epub 2001 Jun 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11395509 11395509]
[[Category: Arsenite-transporting ATPase]]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Gatti, D.L.]]
[[Category: Gatti DL]]
[[Category: Radaev, S.]]
[[Category: Radaev S]]
[[Category: Rosen, B.P.]]
[[Category: Rosen BP]]
[[Category: Zhou, T.]]
[[Category: Zhou T]]
[[Category: ADP]]
[[Category: ANP]]
[[Category: CD]]
[[Category: CL]]
[[Category: MG]]
[[Category: TAS]]
[[Category: amp-pnp]]
[[Category: arsa atpase]]
[[Category: atp binding site]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:25:12 2007''

Latest revision as of 10:35, 7 February 2024

CRYSTAL STRUCTURE OF THE ESCHERICHIA COLI ARSENITE-TRANSLOCATING ATPASE IN COMPLEX WITH AMP-PNPCRYSTAL STRUCTURE OF THE ESCHERICHIA COLI ARSENITE-TRANSLOCATING ATPASE IN COMPLEX WITH AMP-PNP

Structural highlights

1ii9 is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.6Å
Ligands:, , , , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ARSA1_ECOLX Anion-transporting ATPase. Catalyzes the extrusion of the oxyanions arsenite, antimonite and arsenate. Maintenance of a low intracellular concentration of oxyanion produces resistance to the toxic agents.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1ii9, resolution 2.60Å

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