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[[Image:1iib.gif|left|200px]]<br />
<applet load="1iib" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1iib, resolution 1.8&Aring;" />
'''CRYSTAL STRUCTURE OF IIBCELLOBIOSE FROM ESCHERICHIA COLI'''<br />


==Overview==
==CRYSTAL STRUCTURE OF IIBCELLOBIOSE FROM ESCHERICHIA COLI==
BACKGROUND:. The bacterial phosphoenolpyruvate-dependent, phosphotransferase system (PTS) mediates the energy-driven uptake of, carbohydrates and their concomitant phosphorylation. In addition, the PTS, is intimately involved in the regulation of a variety of metabolic and, transcriptional processes in the bacterium. The multiprotein PTS consists, of a membrane channel and at least four cytoplasmic proteins or protein, domains that sequentially transfer a phosphoryl group from, phosphoenolpyruvate to the transported carbohydrate. Determination of the, three-dimensional structure of the IIB enzymes within the multiprotein, complex would provide insights into the mechanisms by which they promote, efficient transport by the membrane channel IIC protein and phosphorylate, the transported ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9032081 (full description)]]
<StructureSection load='1iib' size='340' side='right'caption='[[1iib]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1iib]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IIB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IIB FirstGlance]. <br>
1IIB is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]]. Active as [[http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69]]. Structure known Active Site: 1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IIB OCA]].
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1iib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iib OCA], [https://pdbe.org/1iib PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1iib RCSB], [https://www.ebi.ac.uk/pdbsum/1iib PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1iib ProSAT]</span></td></tr>
==Reference==
</table>
The structure of an energy-coupling protein from bacteria, IIBcellobiose, reveals similarity to eukaryotic protein tyrosine phosphatases., van Montfort RL, Pijning T, Kalk KH, Reizer J, Saier MH Jr, Thunnissen MM, Robillard GT, Dijkstra BW, Structure. 1997 Feb 15;5(2):217-25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9032081 9032081]
== Function ==
[[Category: Escherichia coli]]
[https://www.uniprot.org/uniprot/PTQB_ECOLI PTQB_ECOLI] The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in N,N'-diacetylchitobiose transport.<ref>PMID:10913117</ref>
[[Category: Protein-N(pi)-phosphohistidine--sugar phosphotransferase]]
== Evolutionary Conservation ==
[[Category: Single protein]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Dijkstra, B.W.]]
Check<jmol>
[[Category: Kalk, K.H.]]
  <jmolCheckbox>
[[Category: Montfort, R.L.M.Van.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ii/1iib_consurf.spt"</scriptWhenChecked>
[[Category: Pijning, T.]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: Reizer, J.]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: Robillard, G.T.]]
  </jmolCheckbox>
[[Category: Saier, M.H.]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1iib ConSurf].
[[Category: Thunnissen, M.M.G.M.]]
<div style="clear:both"></div>
[[Category: cysteine phosphorylation]]
== References ==
[[Category: iib enzymes]]
<references/>
[[Category: phosphoenolpyruvate dependent phosphotransferase system]]
__TOC__
[[Category: phosphotransferase]]
</StructureSection>
 
[[Category: Escherichia coli K-12]]
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:00:37 2007''
[[Category: Large Structures]]
[[Category: Dijkstra BW]]
[[Category: Kalk KH]]
[[Category: Pijning T]]
[[Category: Reizer J]]
[[Category: Robillard GT]]
[[Category: Saier MH]]
[[Category: Thunnissen MMGM]]
[[Category: Van Montfort RLM]]

Latest revision as of 10:35, 7 February 2024

CRYSTAL STRUCTURE OF IIBCELLOBIOSE FROM ESCHERICHIA COLICRYSTAL STRUCTURE OF IIBCELLOBIOSE FROM ESCHERICHIA COLI

Structural highlights

1iib is a 2 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.8Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PTQB_ECOLI The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in N,N'-diacetylchitobiose transport.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Keyhani NO, Wang LX, Lee YC, Roseman S. The chitin disaccharide, N,N'-diacetylchitobiose, is catabolized by Escherichia coli and is transported/phosphorylated by the phosphoenolpyruvate:glycose phosphotransferase system. J Biol Chem. 2000 Oct 20;275(42):33084-90. PMID:10913117 doi:http://dx.doi.org/10.1074/jbc.M001043200

1iib, resolution 1.80Å

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