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| [[Image:1ifd.gif|left|200px]]<br /><applet load="1ifd" size="450" color="white" frame="true" align="right" spinBox="true"
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| caption="1ifd, resolution 4.00Å" />
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| '''MODEL-BUILDING STUDIES OF INOVIRUS: GENETIC VARIATIONS ON A GEOMETRIC THEME'''<br />
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| ==Overview== | | ==MODEL-BUILDING STUDIES OF INOVIRUS: GENETIC VARIATIONS ON A GEOMETRIC THEME== |
| Inovirus (filamentous bacteriophage) is a simple system for studying the, rules by which protein primary structure (amino acid sequence) controls, secondary and higher order structure, and thereby function. The virus, occurs naturally as a number of different strains with similar secondary, and higher order structure, but the protein subunit that assembles to form, the virion coat has quite different primary structures in different virus, strains. Despite these differences in primary structure, the subunits of, all strains have much the same size, about 50 residues, which are, distributed by type in much the same way into three domains of primary, structure: a collection of acidic residues in the N-terminal region, a, hydrophobic domain of about 19 residues near the middle, and a collection, of basic residues near the C-terminus. Each subunit can be closely, approximated by an alpha-helix with its long axis roughly parallel to the, fibre axis, sloping from large to small radius in the virion and, interleaving between subunits in the next turn or level. The acidic, residues near the N-terminus of the subunit face outwards on the virion, surface, and explain the low isoelectric point of the virion; the basic, residues near the C-terminus face inwards, where they neutralize the, charge on the DNA at the core of the virion; and the hydrophobic central, domain is involved in interactions which bind neighbouring subunits., Detailed X-ray fibre diffraction analysis of one strain gives the subunit, structure. Comparative model-building studies of different strains, illustrate the common structural principles.
| | <StructureSection load='1ifd' size='340' side='right'caption='[[1ifd]], [[Resolution|resolution]] 4.00Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[1ifd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_fd Enterobacteria phage fd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IFD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IFD FirstGlance]. <br> |
| 1IFD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phage_phi3t Phage phi3t]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IFD OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Fiber diffraction, [[Resolution|Resolution]] 4Å</td></tr> |
| | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ifd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ifd OCA], [https://pdbe.org/1ifd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ifd RCSB], [https://www.ebi.ac.uk/pdbsum/1ifd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ifd ProSAT]</span></td></tr> |
| ==Reference== | | </table> |
| Model-building studies of Inovirus: genetic variations on a geometric theme., Marvin DA, Int J Biol Macromol. 1990 Apr;12(2):125-38. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2078529 2078529]
| | == Function == |
| [[Category: Phage phi3t]] | | [https://www.uniprot.org/uniprot/CAPSD_BPFD CAPSD_BPFD] Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity). |
| [[Category: Single protein]] | | __TOC__ |
| [[Category: Marvin, D.A.]] | | </StructureSection> |
| [[Category: helical virus]] | | [[Category: Enterobacteria phage fd]] |
| [[Category: virus]] | | [[Category: Large Structures]] |
| | | [[Category: Marvin DA]] |
| ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:21:46 2007''
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