1i4w: Difference between revisions

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New page: left|200px<br /><applet load="1i4w" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i4w, resolution 2.60Å" /> '''THE CRYSTAL STRUCTUR...
 
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[[Image:1i4w.gif|left|200px]]<br /><applet load="1i4w" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1i4w, resolution 2.60&Aring;" />
'''THE CRYSTAL STRUCTURE OF THE TRANSCRIPTION FACTOR SC-MTTFB OFFERS INTRIGUING INSIGHTS INTO MITOCHONDRIAL TRANSCRIPTION'''<br />


==Overview==
==THE CRYSTAL STRUCTURE OF THE TRANSCRIPTION FACTOR SC-MTTFB OFFERS INTRIGUING INSIGHTS INTO MITOCHONDRIAL TRANSCRIPTION==
Although it is commonly accepted that binding of mitochondrial, transcription factor sc-mtTFB to the mitochondrial RNA polymerase is, required for specific transcription initiation in Saccharomyces, cerevisiae, its precise role has remained undefined. In the present work, the crystal structure of sc-mtTFB has been determined to 2.6 A resolution., The protein consists of two domains, an N-terminal alpha/beta-domain and a, smaller domain made up of four alpha-helices. Contrary to previous, predictions, sc-mtTFB does not resemble Escherichia coli sigma-factors but, rather is structurally homologous to rRNA methyltransferase ErmC'. This, suggests that sc-mtTFB functions as an RNA-binding protein, an observation, standing in contradiction to the existing model, which proposed a direct, interaction of sc-mtTFB with the mitochondrial DNA promoter. Based on the, structure, we propose that the promoter specificity region is located on, the mitochondrial RNA polymerase and that binding of sc-mtTFB indirectly, mediates interaction of the core enzyme with the promoter site.
<StructureSection load='1i4w' size='340' side='right'caption='[[1i4w]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1i4w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I4W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I4W FirstGlance]. <br>
1I4W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with XE as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I4W OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=XE:XENON'>XE</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i4w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i4w OCA], [https://pdbe.org/1i4w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i4w RCSB], [https://www.ebi.ac.uk/pdbsum/1i4w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i4w ProSAT]</span></td></tr>
Crystal structure of the transcription factor sc-mtTFB offers insights into mitochondrial transcription., Schubot FD, Chen CJ, Rose JP, Dailey TA, Dailey HA, Wang BC, Protein Sci. 2001 Oct;10(10):1980-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11567089 11567089]
</table>
== Function ==
[https://www.uniprot.org/uniprot/MTF1_YEAST MTF1_YEAST] Mitochondrial transcription factor that confers selective promoter recognition on the core subunit of the yeast mitochondrial RNA polymerase. Interacts with DNA in a non-specific manner.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i4/1i4w_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i4w ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Chen C-J]]
[[Category: Chen, C.J.]]
[[Category: Dailey HA]]
[[Category: Dailey, H.A.]]
[[Category: Dailey TA]]
[[Category: Dailey, T.A.]]
[[Category: Rose JP]]
[[Category: Rose, J.P.]]
[[Category: Schubot FD]]
[[Category: Schubot, F.D.]]
[[Category: Wang B-C]]
[[Category: Wang, B.C.]]
[[Category: XE]]
[[Category: mitochondrial transcription factor]]
[[Category: transcription initiation]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:03:11 2007''

Latest revision as of 10:32, 7 February 2024

THE CRYSTAL STRUCTURE OF THE TRANSCRIPTION FACTOR SC-MTTFB OFFERS INTRIGUING INSIGHTS INTO MITOCHONDRIAL TRANSCRIPTIONTHE CRYSTAL STRUCTURE OF THE TRANSCRIPTION FACTOR SC-MTTFB OFFERS INTRIGUING INSIGHTS INTO MITOCHONDRIAL TRANSCRIPTION

Structural highlights

1i4w is a 1 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.6Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MTF1_YEAST Mitochondrial transcription factor that confers selective promoter recognition on the core subunit of the yeast mitochondrial RNA polymerase. Interacts with DNA in a non-specific manner.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1i4w, resolution 2.60Å

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