1i4w: Difference between revisions

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-[[Image:1i4w.gif|left|200px]]
- {{Structure
+==THE CRYSTAL STRUCTURE OF THE TRANSCRIPTION FACTOR SC-MTTFB OFFERS INTRIGUING INSIGHTS INTO MITOCHONDRIAL TRANSCRIPTION==
-|PDB= 1i4w |SIZE=350|CAPTION= <scene name='initialview01'>1i4w</scene>, resolution 2.60&Aring;
+<StructureSection load='1i4w' size='340' side='right'caption='[[1i4w]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=XE:XENON'>XE</scene>
+<table><tr><td colspan='2'>[[1i4w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I4W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I4W FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
-|GENE= MTF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=XE:XENON'>XE</scene></td></tr>
-}}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i4w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i4w OCA], [https://pdbe.org/1i4w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i4w RCSB], [https://www.ebi.ac.uk/pdbsum/1i4w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i4w ProSAT]</span></td></tr>
+</table>
-'''THE CRYSTAL STRUCTURE OF THE TRANSCRIPTION FACTOR SC-MTTFB OFFERS INTRIGUING INSIGHTS INTO MITOCHONDRIAL TRANSCRIPTION'''
+== Function ==
+[https://www.uniprot.org/uniprot/MTF1_YEAST MTF1_YEAST] Mitochondrial transcription factor that confers selective promoter recognition on the core subunit of the yeast mitochondrial RNA polymerase. Interacts with DNA in a non-specific manner.
+== Evolutionary Conservation ==
-==Overview==
+[[Image:Consurf_key_small.gif|200px|right]]
-Although it is commonly accepted that binding of mitochondrial transcription factor sc-mtTFB to the mitochondrial RNA polymerase is required for specific transcription initiation in Saccharomyces cerevisiae, its precise role has remained undefined. In the present work, the crystal structure of sc-mtTFB has been determined to 2.6 A resolution. The protein consists of two domains, an N-terminal alpha/beta-domain and a smaller domain made up of four alpha-helices. Contrary to previous predictions, sc-mtTFB does not resemble Escherichia coli sigma-factors but rather is structurally homologous to rRNA methyltransferase ErmC'. This suggests that sc-mtTFB functions as an RNA-binding protein, an observation standing in contradiction to the existing model, which proposed a direct interaction of sc-mtTFB with the mitochondrial DNA promoter. Based on the structure, we propose that the promoter specificity region is located on the mitochondrial RNA polymerase and that binding of sc-mtTFB indirectly mediates interaction of the core enzyme with the promoter site.
+Check<jmol>
+  <jmolCheckbox>
-==About this Structure==
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i4/1i4w_consurf.spt"</scriptWhenChecked>
-I4W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I4W OCA].
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
-==Reference==
+  </jmolCheckbox>
-Crystal structure of the transcription factor sc-mtTFB offers insights into mitochondrial transcription., Schubot FD, Chen CJ, Rose JP, Dailey TA, Dailey HA, Wang BC, Protein Sci. 2001 Oct;10(10):1980-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11567089 11567089]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i4w ConSurf].
+<div style="clear:both"></div>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Single protein]]
+[[Category: Chen C-J]]
-[[Category: Chen, C J.]]
+[[Category: Dailey HA]]
-[[Category: Dailey, H A.]]
+[[Category: Dailey TA]]
-[[Category: Dailey, T A.]]
+[[Category: Rose JP]]
-[[Category: Rose, J P.]]
+[[Category: Schubot FD]]
-[[Category: Schubot, F D.]]
+[[Category: Wang B-C]]
-[[Category: Wang, B C.]]
-[[Category: XE]]
-[[Category: mitochondrial transcription factor]]
-[[Category: transcription initiation]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:45:35 2008''