1i27: Difference between revisions

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OCA

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-[[Image:1i27.gif|left|200px]]<br />
-<applet load="1i27" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1i27, resolution 1.02&Aring;" />
-'''CRYSTAL STRUCTURE OF THE C-TERMINAL DOMAIN OF THE RAP74 SUBUNIT OF HUMAN TRANSCRIPTION FACTOR IIF (TFIIF)'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF THE C-TERMINAL DOMAIN OF THE RAP74 SUBUNIT OF HUMAN TRANSCRIPTION FACTOR IIF (TFIIF)==
-The x-ray structure of a C-terminal fragment of the RAP74 subunit of human, transcription factor (TF) IIF has been determined at 1.02-A resolution., The alpha/beta structure is strikingly similar to the globular domain of, linker histone H5 and the DNA-binding domain of hepatocyte nuclear factor, 3gamma (HNF-3gamma), making it a winged-helix protein. The surface, electrostatic properties of this compact domain differ significantly from, those of bona fide winged-helix transcription factors (HNF-3gamma and, RFX1) and from the winged-helix domains found within the RAP30 subunit of, TFIIF and the beta subunit of TFIIE. RAP74 has been shown to interact with, the TFIIF-associated C-terminal domain phosphatase FCP1, and a putative, phosphatase binding site has been identified within the RAP74 winged-helix, domain.
+<StructureSection load='1i27' size='340' side='right'caption='[[1i27]], [[Resolution|resolution]] 1.02&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1i27]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I27 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I27 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.02&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i27 OCA], [https://pdbe.org/1i27 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i27 RCSB], [https://www.ebi.ac.uk/pdbsum/1i27 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i27 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/T2FA_HUMAN T2FA_HUMAN] TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.<ref>PMID:10428810</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i2/1i27_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i27 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-I27 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I27 OCA].
+*[[Transcription initiation factors 3D structures|Transcription initiation factors 3D structures]]
+== References ==
-==Reference==
+<references/>
-Crystal structure of the C-terminal domain of the RAP74 subunit of human transcription factor IIF., Kamada K, De Angelis J, Roeder RG, Burley SK, Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3115-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11248041 11248041]
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Angelis, J.De.]]
+[[Category: Burley SK]]
-[[Category: Burley, S.K.]]
+[[Category: De Angelis J]]
-[[Category: Kamada, K.]]
+[[Category: Kamada K]]
-[[Category: Roeder, R.G.]]
+[[Category: Roeder RG]]
-[[Category: ZN]]
-[[Category: general transcription factor]]
-[[Category: rap30]]
-[[Category: rap74]]
-[[Category: rna polymerase ii]]
-[[Category: tfiif]]
-[[Category: winged-helix domain]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:25:35 2007''