1hvg: Difference between revisions

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-[[Image:1hvg.gif|left|200px]]<br />
-<applet load="1hvg" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1hvg, resolution 3.0&Aring;" />
-'''STRUCTURAL AND ELECTROPHYSIOLOGICAL ANALYSIS OF ANNEXIN V MUTANTS. MUTAGENESIS OF HUMAN ANNEXIN V, AN IN VITRO VOLTAGE-GATED CALCIUM CHANNEL, PROVIDES INFORMATION ABOUT THE STRUCTURAL FEATURES OF THE ION PATHWAY, THE VOLTAGE SENSOR AND THE ION SELECTIVITY FILTER'''<br />
-==Overview==
+==STRUCTURAL AND ELECTROPHYSIOLOGICAL ANALYSIS OF ANNEXIN V MUTANTS. MUTAGENESIS OF HUMAN ANNEXIN V, AN IN VITRO VOLTAGE-GATED CALCIUM CHANNEL, PROVIDES INFORMATION ABOUT THE STRUCTURAL FEATURES OF THE ION PATHWAY, THE VOLTAGE SENSOR AND THE ION SELECTIVITY FILTER==
-Annexin V binds to phospholipids in a calcium-dependent manner and, exhibits calcium channel activity in vitro. We prepared a variety of, mutants yielding information about the structure-function relationship of, the ion channel activity. All mutants were characterized by X-ray, crystallography, electron microscopy and electrophysiological, measurements. Their structures are insignificantly changed whereas their, electrophysiological properties are drastically different. Glu95, located, in the central hydrophilic pore of the molecule, is crucial for the ion, selectivity filter as its exchange leads to reduced calcium and increased, sodium conductance. The removal of Glu17, located on the protein surface, and far from the ion conduction pathway, leads to the appearance of a, second conductance level of 9 pS in addition to the conductance level of, about 30 pS in the wild-type molecule. This was also the case for Glu78, which is part of a weak calcium binding site. The exchange of Glu17 and, Glu78 produced a mutant retaining only the smaller conductance level. We, conclude that these two residues influence the angle between the two, halves of the molecule, which determines the diameter of the ion, conduction pathway, thereby leading to the occurrence of a second, conductance level.
+<StructureSection load='1hvg' size='340' side='right'caption='[[1hvg]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1hvg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HVG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HVG FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hvg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hvg OCA], [https://pdbe.org/1hvg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hvg RCSB], [https://www.ebi.ac.uk/pdbsum/1hvg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hvg ProSAT]</span></td></tr>
+</table>
+== Disease ==
+[https://www.uniprot.org/uniprot/ANXA5_HUMAN ANXA5_HUMAN] Defects in ANXA5 are associated with susceptibility to pregnancy loss, recurrent, type 3 (RPRGL3) [MIM:[https://omim.org/entry/614391 614391]. A common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions.<ref>PMID:17339269</ref>
+== Function ==
+[https://www.uniprot.org/uniprot/ANXA5_HUMAN ANXA5_HUMAN] This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hv/1hvg_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hvg ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-HVG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HVG OCA].
+*[[Annexin 3D structures|Annexin 3D structures]]
+== References ==
-==Reference==
+<references/>
-Structural and electrophysiological analysis of annexin V mutants. Mutagenesis of human annexin V, an in vitro voltage-gated calcium channel, provides information about the structural features of the ion pathway, the voltage sensor and the ion selectivity filter., Burger A, Voges D, Demange P, Perez CR, Huber R, Berendes R, J Mol Biol. 1994 Apr 8;237(4):479-99. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8151707 8151707]
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Burger, A.]]
+[[Category: Burger A]]
-[[Category: Huber, R.]]
+[[Category: Huber R]]
-[[Category: calcium/phospholipid binding]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 17:23:17 2007''