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| ==CRYSTAL STRUCTURE OF THE N-TERMINAL DOMAIN OF THE TYROSINE PHOSPHATASE YOPH FROM YERSINIA PESTIS.== | | ==CRYSTAL STRUCTURE OF THE N-TERMINAL DOMAIN OF THE TYROSINE PHOSPHATASE YOPH FROM YERSINIA PESTIS.== |
| <StructureSection load='1huf' size='340' side='right' caption='[[1huf]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='1huf' size='340' side='right'caption='[[1huf]], [[Resolution|resolution]] 2.00Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[1huf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pestis"_(lehmann_and_neumann_1896)_migula_1900 "bacillus pestis" (lehmann and neumann 1896) migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HUF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HUF FirstGlance]. <br> | | <table><tr><td colspan='2'>[[1huf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HUF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HUF FirstGlance]. <br> |
| </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ypt|1ypt]]</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">YOPH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 "Bacillus pestis" (Lehmann and Neumann 1896) Migula 1900])</td></tr>
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1huf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1huf OCA], [https://pdbe.org/1huf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1huf RCSB], [https://www.ebi.ac.uk/pdbsum/1huf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1huf ProSAT]</span></td></tr> |
| <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>
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| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1huf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1huf OCA], [http://pdbe.org/1huf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1huf RCSB], [http://www.ebi.ac.uk/pdbsum/1huf PDBsum]</span></td></tr> | |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/O68720_YERPE O68720_YERPE] |
| Yersinia pestis, the causative agent of bubonic plague, injects effector proteins into the cytosol of mammalian cells that enable the bacterium to evade the immune response of the infected organism by interfering with eukaryotic signal transduction pathways. YopH is a modular effector composed of a C-terminal protein tyrosine phosphatase (PTPase) domain and a multifunctional N-terminal domain that not only orchestrates the secretion and translocation of YopH into eukaryotic cells but also binds tyrosine-phosphorylated target proteins to mediate substrate recognition. The crystal structure of the N-terminal domain of YopH (YopH(N); residues 1-130) has been determined at 2.0 A resolution. The amino-acid sequences that target YopH for secretion from the bacterium and translocation into eukaryotic cells form integral parts of this compactly folded domain. The structure of YopH(N) bears no resemblance to eukaryotic phosphotyrosine-binding domains, nor is it reminiscent of any known fold. Residues that have been implicated in phosphotyrosine-dependent protein binding are clustered together on one face of YopH(N), but the structure does not suggest a mechanism for protein-phosphotyrosine recognition.
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| Structure of the N-terminal domain of Yersinia pestis YopH at 2.0 A resolution.,Evdokimov AG, Tropea JE, Routzahn KM, Copeland TD, Waugh DS Acta Crystallogr D Biol Crystallogr. 2001 Jun;57(Pt 6):793-9. Epub 2001, May 25. PMID:11375498<ref>PMID:11375498</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 1huf" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| *[[Tyrosine phosphatase|Tyrosine phosphatase]] | | *[[Tyrosine phosphatase 3D structures|Tyrosine phosphatase 3D structures]] |
| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Protein-tyrosine-phosphatase]] | | [[Category: Large Structures]] |
| [[Category: Evdokimov, A G]] | | [[Category: Yersinia pestis]] |
| [[Category: Waugh, D S]] | | [[Category: Evdokimov AG]] |
| [[Category: Beta hairpin]] | | [[Category: Waugh DS]] |
| [[Category: Helical bundle]]
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| [[Category: Hydrolase]]
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