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| [[Image:1hrd.gif|left|200px]]
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| {{Structure
| | ==GLUTAMATE DEHYDROGENASE== |
| |PDB= 1hrd |SIZE=350|CAPTION= <scene name='initialview01'>1hrd</scene>, resolution 1.96Å
| | <StructureSection load='1hrd' size='340' side='right'caption='[[1hrd]], [[Resolution|resolution]] 1.96Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND=
| | <table><tr><td colspan='2'>[[1hrd]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_symbiosum Clostridium symbiosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HRD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HRD FirstGlance]. <br> |
| |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate_dehydrogenase Glutamate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.1.2 1.4.1.2] </span>
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.96Å</td></tr> |
| |GENE=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hrd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hrd OCA], [https://pdbe.org/1hrd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hrd RCSB], [https://www.ebi.ac.uk/pdbsum/1hrd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hrd ProSAT]</span></td></tr> |
| |DOMAIN=
| | </table> |
| |RELATEDENTRY=
| | == Function == |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hrd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hrd OCA], [http://www.ebi.ac.uk/pdbsum/1hrd PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hrd RCSB]</span>
| | [https://www.uniprot.org/uniprot/DHE2_CLOSY DHE2_CLOSY] |
| }}
| | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> |
| | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hr/1hrd_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> |
| | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hrd ConSurf]. |
| | <div style="clear:both"></div> |
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| '''GLUTAMATE DEHYDROGENASE'''
| | ==See Also== |
| | | *[[Glutamate dehydrogenase 3D structures|Glutamate dehydrogenase 3D structures]] |
| | | __TOC__ |
| ==Overview== | | </StructureSection> |
| BACKGROUND: The hyperthermophile Pyrococcus furiosus is one of the most thermostable organisms known, with an optimum growth temperature of 100 degrees C. The proteins from this organism display extreme thermostability. We have undertaken the structure determination of glutamate dehydrogenase from P. furiosus in order to gain further insights into the relationship between molecular structure and thermal stability. RESULTS: The structure of P. furiosus glutamate dehydrogenase, a homohexameric enzyme, has been determined at 2.2 A resolution and compared with the structure of glutamate dehydrogenase from the mesophile Clostridium symbiosum. CONCLUSIONS: Comparison of the structures of these two enzymes has revealed one major difference: the structure of the hyperthermophilic enzyme contains a striking series of ion-pair networks on the surface of the protein subunits and buried at both interdomain and intersubunit interfaces. We propose that the formation of such extended networks may represent a major stabilizing feature associated with the adaptation of enzymes to extreme temperatures.
| | [[Category: Large Structures]] |
| | | [[Category: Baker PJ]] |
| ==About this Structure==
| | [[Category: Britton KL]] |
| 1HRD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_symbiosum Clostridium symbiosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HRD OCA].
| | [[Category: Rice DW]] |
| | | [[Category: Stillman TJ]] |
| ==Reference==
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| The structure of Pyrococcus furiosus glutamate dehydrogenase reveals a key role for ion-pair networks in maintaining enzyme stability at extreme temperatures., Yip KS, Stillman TJ, Britton KL, Artymiuk PJ, Baker PJ, Sedelnikova SE, Engel PC, Pasquo A, Chiaraluce R, Consalvi V, Structure. 1995 Nov 15;3(11):1147-58. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8591026 8591026]
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| [[Category: Clostridium symbiosum]] | |
| [[Category: Glutamate dehydrogenase]]
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| [[Category: Single protein]]
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| [[Category: Baker, P J.]] | |
| [[Category: Britton, K L.]] | |
| [[Category: Rice, D W.]] | |
| [[Category: Stillman, T J.]] | |
| [[Category: nad]]
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| [[Category: oxidoreductase]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:09:25 2008''
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