1hlw: Difference between revisions

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New page: left|200px<br /><applet load="1hlw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hlw, resolution 1.90Å" /> '''STRUCTURE OF THE H12...
 
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[[Image:1hlw.jpg|left|200px]]<br /><applet load="1hlw" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1hlw, resolution 1.90&Aring;" />
'''STRUCTURE OF THE H122A MUTANT OF THE NUCLEOSIDE DIPHOSPHATE KINASE'''<br />


==Overview==
==STRUCTURE OF THE H122A MUTANT OF THE NUCLEOSIDE DIPHOSPHATE KINASE==
We have explored the ability of a nucleoside diphosphate kinase (NDPK), mutant in which the nucleophilic histidine has been replaced by glycine, (H122G) to transfer phosphate from ATP to alcohols of varying pK(a), size, shape, and polarity. This cavity mutant does indeed act as a primitive, alcohol kinase. The rate of its phosphoryl transfer to alcohols varies, considerably, with values spanning a DeltaDeltaG(double dagger) range of 4, kcal/mol, whereas the alcohols have very similar intrinsic reactivities., Analysis of these results suggests that the ability to carry out, phosphoryl transfer within the cavity is not a simple function of being, small enough to enter the cavity, but rather is a complex function of, steric, solvation, entropic, van der Waals packing, and electrostatic, properties of the alcohol. In addition, large differences are observed, between the reactivities of alcohols within the nucleophile cavity of, H122G and the reactivities of the same alcohols within the nucleophile, cavity of H122A, a mutant NDPK that differs from H122G by a single methyl, group within the cavity. The crystal structures of the two cavity mutants, are very similar to one another and to wild-type NDPK, providing no, evidence for a structurally perturbed active site. The differences in, reactivity between the two mutant proteins illustrate a fundamental, limitation of energetic analysis from site-directed mutagenesis: although, removal of a side chain is generally considered to be a conservative, change, the energetic effects of any given mutation are inextricably, linked to the molecular properties of the created cavity and the, surrounding protein environment.
<StructureSection load='1hlw' size='340' side='right'caption='[[1hlw]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1hlw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HLW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HLW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hlw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hlw OCA], [https://pdbe.org/1hlw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hlw RCSB], [https://www.ebi.ac.uk/pdbsum/1hlw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hlw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NDKC_DICDI NDKC_DICDI]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hl/1hlw_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hlw ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1HLW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Active as [http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HLW OCA].
*[[Nucleoside diphosphate kinase 3D structures|Nucleoside diphosphate kinase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Chemical rescue of phosphoryl transfer in a cavity mutant: a cautionary tale for site-directed mutagenesis., Admiraal SJ, Meyer P, Schneider B, Deville-Bonne D, Janin J, Herschlag D, Biochemistry. 2001 Jan 16;40(2):403-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11148034 11148034]
[[Category: Dictyostelium discoideum]]
[[Category: Dictyostelium discoideum]]
[[Category: Nucleoside-diphosphate kinase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Admiraal SJ]]
[[Category: Admiraal, S.J.]]
[[Category: Deville-Bonne D]]
[[Category: Deville-Bonne, D.]]
[[Category: Herschlag D]]
[[Category: Herschlag, D.]]
[[Category: Janin J]]
[[Category: Janin, J.]]
[[Category: Meyer P]]
[[Category: Meyer, P.]]
[[Category: Schneider B]]
[[Category: Schneider, B.]]
[[Category: atp-binding]]
[[Category: kinase]]
[[Category: phosphotransferase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:38:25 2007''

Latest revision as of 10:28, 7 February 2024

STRUCTURE OF THE H122A MUTANT OF THE NUCLEOSIDE DIPHOSPHATE KINASESTRUCTURE OF THE H122A MUTANT OF THE NUCLEOSIDE DIPHOSPHATE KINASE

Structural highlights

1hlw is a 1 chain structure with sequence from Dictyostelium discoideum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.9Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NDKC_DICDI

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1hlw, resolution 1.90Å

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OCA
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