1hjp: Difference between revisions

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New page: left|200px<br /><applet load="1hjp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hjp, resolution 2.5Å" /> '''HOLLIDAY JUNCTION BIN...
 
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[[Image:1hjp.jpg|left|200px]]<br /><applet load="1hjp" size="450" color="white" frame="true" align="right" spinBox="true"
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'''HOLLIDAY JUNCTION BINDING PROTEIN RUVA FROM E. COLI'''<br />


==Overview==
==HOLLIDAY JUNCTION BINDING PROTEIN RUVA FROM E. COLI==
BACKGROUND: Homologous recombination is crucial for genetic diversity and, repairing damaged chromosomes. In Escherichia coli cells, the RuvA, RuvB, and RuvC proteins participate in the processing of an important, intermediate, the Holliday junction. The RuvA-RuvB protein complex, facilitates branch migration of the junction, depending on ATP hydrolysis., The atomic structure of RuvA should enable critical questions to be, addressed about its specific interactions with the Holliday junction and, the RuvB protein. RESULTS: The crystal structure of RuvA shows the, tetrameric molecules with a fourfold axis at the center. Each subunit, consists of three distinct domains, some of which contain important, secondary structure elements for DNA binding. Together with the detailed, structural information, the biochemical assays of various mutant RuvA, proteins and domains, isolated by partial proteolysis, allowed us to, define the functional roles of these domains in Holliday junction binding, and the RuvB interaction. CONCLUSIONS: The RuvA molecule is formed by four, identical subunits, each with three domains, I, II and III. The locations, of the putative DNA-binding motifs define an interface between the DNA and, the Holliday junction. Domain III is weakly attached to the core region, comprising domains I and II; the core domains can form a tetramer in the, absence of domain III. Functional analyses of the mutant proteins and the, partial digestion products, including Holliday junction binding and, branch-migration assays, revealed that domain III and the preceding loop, are crucial for RuvB binding and branch migration, although this region is, not required for the junction-DNA binding.
<StructureSection load='1hjp' size='340' side='right'caption='[[1hjp]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1hjp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HJP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hjp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hjp OCA], [https://pdbe.org/1hjp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hjp RCSB], [https://www.ebi.ac.uk/pdbsum/1hjp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hjp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RUVA_ECOLI RUVA_ECOLI] The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB. Binds both single- and double-stranded DNA (dsDNA). Binds preferentially to supercoiled rather than to relaxed dsDNA.<ref>PMID:8433990</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hj/1hjp_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hjp ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1HJP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HJP OCA].
*[[Helicase 3D structures|Helicase 3D structures]]
 
== References ==
==Reference==
<references/>
Functional analyses of the domain structure in the Holliday junction binding protein RuvA., Nishino T, Ariyoshi M, Iwasaki H, Shinagawa H, Morikawa K, Structure. 1998 Jan 15;6(1):11-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9493263 9493263]
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Ariyoshi, M.]]
[[Category: Ariyoshi M]]
[[Category: Iwasaki, H.]]
[[Category: Iwasaki H]]
[[Category: Morikawa, K.]]
[[Category: Morikawa K]]
[[Category: Nishino, T.]]
[[Category: Nishino T]]
[[Category: Shinagawa, H.]]
[[Category: Shinagawa H]]
[[Category: dna recombination]]
[[Category: dna repair]]
[[Category: holliday junction binding]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:36:47 2007''

Latest revision as of 10:28, 7 February 2024

HOLLIDAY JUNCTION BINDING PROTEIN RUVA FROM E. COLIHOLLIDAY JUNCTION BINDING PROTEIN RUVA FROM E. COLI

Structural highlights

1hjp is a 1 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.5Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RUVA_ECOLI The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB. Binds both single- and double-stranded DNA (dsDNA). Binds preferentially to supercoiled rather than to relaxed dsDNA.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Tsaneva IR, Muller B, West SC. RuvA and RuvB proteins of Escherichia coli exhibit DNA helicase activity in vitro. Proc Natl Acad Sci U S A. 1993 Feb 15;90(4):1315-9. PMID:8433990

1hjp, resolution 2.50Å

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