1gle: Difference between revisions

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New page: left|200px<br /><applet load="1gle" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gle, resolution 2.94Å" /> '''CATION PROMOTED ASSO...
 
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'''CATION PROMOTED ASSOCIATION (CPA) OF A REGULATORY AND TARGET PROTEIN IS CONTROLLED BY PHOSPHORYLATION'''<br />


==Overview==
==CATION PROMOTED ASSOCIATION (CPA) OF A REGULATORY AND TARGET PROTEIN IS CONTROLLED BY PHOSPHORYLATION==
A central question in molecular biology concerns the means by which a, regulatory protein recognizes different targets. IIIGlc, the, glucose-specific phosphocarrier protein of the bacterial, phosphotransferase system, is also the central regulatory element of the, PTS. Binding of unphosphorylated IIIGlc inhibits several non-PTS proteins, but there is little or no sequence similarity between IIIGlc binding sites, on different target proteins. The crystal structure of Escherichia coli, IIIGlc bound to one of its regulatory targets, glycerol kinase, has been, refined at 2.6-A resolution in the presence of products, adenosine, diphosphate and glycerol 3-phosphate. Structural and kinetic analyses show, that the complex of IIIGlc with glycerol kinase creates an intermolecular, Zn(II) binding site with ligation identical to that of the zinc peptidase, thermolysin. The zinc is coordinated by the two active-site histidines of, IIIGlc, a glutamate of glycerol kinase, and a water molecule. Zn(II) at, 0.01 and 0.1 mM decreases the Ki of IIIGlc for glycerol kinase by factors, of about 15 and 60, respectively. The phosphorylation of one of the, histidines of IIIGlc, in its alternative role as phosphocarrier, provides, an elegant means of controlling the cation-enhanced protein-protein, regulatory interaction. The need for the target protein to supply only one, metal ligand may account for the lack of sequence similarity among the, regulatory targets of IIIGlc.
<StructureSection load='1gle' size='340' side='right'caption='[[1gle]], [[Resolution|resolution]] 2.94&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1gle]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GLE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GLE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.94&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=G3H:GLYCERALDEHYDE-3-PHOSPHATE'>G3H</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gle OCA], [https://pdbe.org/1gle PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gle RCSB], [https://www.ebi.ac.uk/pdbsum/1gle PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gle ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PTGA_ECOLI PTGA_ECOLI] The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gl/1gle_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gle ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1GLE is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN, G3H and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GLE OCA].
*[[Glycerol kinase|Glycerol kinase]]
 
__TOC__
==Reference==
</StructureSection>
Cation-promoted association of a regulatory and target protein is controlled by protein phosphorylation., Feese M, Pettigrew DW, Meadow ND, Roseman S, Remington SJ, Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3544-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8170944 8170944]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Protein-N(pi)-phosphohistidine--sugar phosphotransferase]]
[[Category: Feese MD]]
[[Category: Feese, M.D.]]
[[Category: Meadow ND]]
[[Category: Meadow, N.D.]]
[[Category: Pettigrew DW]]
[[Category: Pettigrew, D.W.]]
[[Category: Remington SJ]]
[[Category: Remington, S.J.]]
[[Category: Roseman S]]
[[Category: Roseman, S.]]
[[Category: ADP]]
[[Category: G3H]]
[[Category: ZN]]
[[Category: phosphotransferase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:08:47 2007''

Latest revision as of 10:25, 7 February 2024

CATION PROMOTED ASSOCIATION (CPA) OF A REGULATORY AND TARGET PROTEIN IS CONTROLLED BY PHOSPHORYLATIONCATION PROMOTED ASSOCIATION (CPA) OF A REGULATORY AND TARGET PROTEIN IS CONTROLLED BY PHOSPHORYLATION

Structural highlights

1gle is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.94Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PTGA_ECOLI The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1gle, resolution 2.94Å

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