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| <!--
| | ==GDP-FUCOSE SYNTHETASE FROM E. COLI== |
| The line below this paragraph, containing "STRUCTURE_1gfs", creates the "Structure Box" on the page.
| | <StructureSection load='1gfs' size='340' side='right'caption='[[1gfs]], [[Resolution|resolution]] 2.20Å' scene=''> |
| You may change the PDB parameter (which sets the PDB file loaded into the applet)
| | == Structural highlights == |
| or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| | <table><tr><td colspan='2'>[[1gfs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GFS FirstGlance]. <br> |
| or leave the SCENE parameter empty for the default display.
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| -->
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gfs OCA], [https://pdbe.org/1gfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gfs RCSB], [https://www.ebi.ac.uk/pdbsum/1gfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gfs ProSAT]</span></td></tr> |
| {{STRUCTURE_1gfs| PDB=1gfs | SCENE= }}
| | </table> |
| | | == Function == |
| '''GDP-FUCOSE SYNTHETASE FROM E. COLI'''
| | [https://www.uniprot.org/uniprot/FCL_ECOLI FCL_ECOLI] Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.<ref>PMID:9473059</ref> <ref>PMID:10480878</ref> <ref>PMID:11021971</ref> |
| | | == Evolutionary Conservation == |
| | | [[Image:Consurf_key_small.gif|200px|right]] |
| ==Overview== | | Check<jmol> |
| Background:. In all species examined, GDP-fucose is synthesized from GDP-mannose in a three-step reaction catalyzed by two enzymes, GDP-mannose 4,6 dehydratase and a dual function 3, 5-epimerase-4-reductase named GDP-fucose synthetase. In this latter aspect fucose biosynthesis differs from that of other deoxy and dideoxy sugars, in which the epimerase and reductase activities are present as separate enzymes. Defects in GDP-fucose biosynthesis have been shown to affect nodulation in bacteria, stem development in plants, and are associated with the immune defect leukocyte adhesion deficiency type II in humans. Results:. We have determined the structure of GDP-fucose synthetase from Escherichia coli at 2.2 A resolution. The structure of GDP-fucose synthetase is closely related to that of UDP-galactose 4-epimerase and more distantly to other members of the short-chain dehydrogenase/reductase family. We have also determined the structures of the binary complexes of GDP-fucose synthetase with its substrate NADPH and its product NADP+. The nicotinamide cofactors bind in the syn and anti conformations, respectively. Conclusions:. GDP-fucose synthetase binds its substrate, NADPH, in the proper orientation (syn) for transferring the 4-pro-S hydride of the nicotinamide. We have observed a single binding site in GDP-fucose synthetase for the second substrate, GDP-4-keto,6-deoxy-mannose. This implies that both the epimerization and reduction reactions occur at the same site in the enzyme. As is the case for all members of the short-chain family of dehydrogenase/reductases, GDP-fucose synthetase retains the Ser-Tyr-Lys catalytic triad. We propose that this catalytic triad functions in a mechanistically equivalent manner in both the epimerization and reduction reactions. Additionally, the X-ray structure has allowed us to identify other residues that are potentially required for substrate binding and catalysis.
| | <jmolCheckbox> |
| | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gf/1gfs_consurf.spt"</scriptWhenChecked> |
| ==About this Structure== | | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| 1GFS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GFS OCA].
| | <text>to colour the structure by Evolutionary Conservation</text> |
| | | </jmolCheckbox> |
| ==Reference== | | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gfs ConSurf]. |
| GDP-fucose synthetase from Escherichia coli: structure of a unique member of the short-chain dehydrogenase/reductase family that catalyzes two distinct reactions at the same active site., Somers WS, Stahl ML, Sullivan FX, Structure. 1998 Dec 15;6(12):1601-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9862812 9862812]
| | <div style="clear:both"></div> |
| [[Category: Escherichia coli]] | | == References == |
| [[Category: Single protein]] | | <references/> |
| [[Category: Somers, W S.]] | | __TOC__ |
| [[Category: Stahl, M L.]] | | </StructureSection> |
| [[Category: Sullivan, F X.]] | | [[Category: Escherichia coli K-12]] |
| [[Category: Epimerase-reductase]] | | [[Category: Large Structures]] |
| [[Category: Gdp-fucose]] | | [[Category: Somers WS]] |
| [[Category: Lipopolysaccharide biosynthesis]] | | [[Category: Stahl ML]] |
| [[Category: Nadp]] | | [[Category: Sullivan FX]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:30:38 2008''
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