1g99: Difference between revisions

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-[[Image:1g99.gif|left|200px]]
-<!--
+==AN ANCIENT ENZYME: ACETATE KINASE FROM METHANOSARCINA THERMOPHILA==
-The line below this paragraph, containing "STRUCTURE_1g99", creates the "Structure Box" on the page.
+<StructureSection load='1g99' size='340' side='right'caption='[[1g99]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1g99]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_thermophila Methanosarcina thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G99 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G99 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-{{STRUCTURE_1g99|  PDB=1g99  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g99 OCA], [https://pdbe.org/1g99 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g99 RCSB], [https://www.ebi.ac.uk/pdbsum/1g99 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g99 ProSAT]</span></td></tr>
+</table>
-'''AN ANCIENT ENZYME: ACETATE KINASE FROM METHANOSARCINA THERMOPHILA'''
+== Function ==
+[https://www.uniprot.org/uniprot/ACKA_METTE ACKA_METTE] Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction. Can also phosphorylate propionate, but has very low activity toward butyrate.<ref>PMID:15774882</ref>
+== Evolutionary Conservation ==
-==Overview==
+[[Image:Consurf_key_small.gif|200px|right]]
-Acetate kinase, an enzyme widely distributed in the Bacteria and Archaea domains, catalyzes the phosphorylation of acetate. We have determined the three-dimensional structure of Methanosarcina thermophila acetate kinase bound to ADP through crystallography. As we previously predicted, acetate kinase contains a core fold that is topologically identical to that of the ADP-binding domains of glycerol kinase, hexokinase, the 70-kDa heat shock cognate (Hsc70), and actin. Numerous charged active-site residues are conserved within acetate kinases, but few are conserved within the phosphotransferase superfamily. The identity of the points of insertion of polypeptide segments into the core fold of the superfamily members indicates that the insertions existed in the common ancestor of the phosphotransferases. Another remarkable shared feature is the unusual, epsilon conformation of the residue that directly precedes a conserved glycine residue (Gly-331 in acetate kinase) that binds the alpha-phosphate of ADP. Structural, biochemical, and geochemical considerations indicate that an acetate kinase may be the ancestral enzyme of the ASKHA (acetate and sugar kinases/Hsc70/actin) superfamily of phosphotransferases.
+Check<jmol>
+  <jmolCheckbox>
-==About this Structure==
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g9/1g99_consurf.spt"</scriptWhenChecked>
-G99 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanosarcina_thermophila Methanosarcina thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G99 OCA].
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
-==Reference==
+  </jmolCheckbox>
-Urkinase: structure of acetate kinase, a member of the ASKHA superfamily of phosphotransferases., Buss KA, Cooper DR, Ingram-Smith C, Ferry JG, Sanders DA, Hasson MS, J Bacteriol. 2001 Jan;183(2):680-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11133963 11133963]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g99 ConSurf].
-[[Category: Acetate kinase]]
+<div style="clear:both"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Methanosarcina thermophila]]
-[[Category: Single protein]]
+[[Category: Buss KA]]
-[[Category: Buss, K A.]]
+[[Category: Cooper DR]]
-[[Category: Cooper, D R.]]
+[[Category: Ferry JG]]
-[[Category: Ferry, J G.]]
+[[Category: Hasson MS]]
-[[Category: Hasson, M S.]]
+[[Category: Ingram-Smith C]]
-[[Category: Ingram-Smith, C.]]
+[[Category: Sanders DA]]
-[[Category: Sanders, D A.]]
-[[Category: Alpha/beta]]
-[[Category: Conserved epsilon conformation]]
-[[Category: Hsc70]]
-[[Category: Two similar domain]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 17:18:19 2008''