1g7s: Difference between revisions

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OCA

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-[[Image:1g7s.jpg|left|200px]]<br /><applet load="1g7s" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1g7s, resolution 2.&Aring;" />
-'''X-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B COMPLEXED WITH GDP'''<br />
-==Overview==
+==X-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B COMPLEXED WITH GDP==
-X-ray structures of the universal translation initiation factor IF2/eIF5B, have been determined in three states: free enzyme, inactive IF2/eIF5B.GDP, and active IF2/eIF5B.GTP. The "chalice-shaped" enzyme is a GTPase that, facilitates ribosomal subunit joining and Met-tRNA(i) binding to ribosomes, in all three kingdoms of life. The conserved core of IF2/eIF5B consists of, an N-terminal G domain (I) plus an EF-Tu-type beta barrel (II), followed, by a novel alpha/beta/alpha-sandwich (III) connected via an alpha helix to, a second EF-Tu-type beta barrel (IV). Structural comparisons reveal a, molecular lever, which amplifies a modest conformational change in the, Switch 2 region of the G domain induced by Mg(2+)/GTP binding over a, distance of 90 A from the G domain active center to domain IV. Mechanisms, of GTPase function and ribosome binding are discussed.
+<StructureSection load='1g7s' size='340' side='right'caption='[[1g7s]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1g7s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G7S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G7S FirstGlance]. <br>
-G7S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus] with GDP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G7S OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g7s OCA], [https://pdbe.org/1g7s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g7s RCSB], [https://www.ebi.ac.uk/pdbsum/1g7s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g7s ProSAT]</span></td></tr>
-X-Ray structures of the universal translation initiation factor IF2/eIF5B: conformational changes on GDP and GTP binding., Roll-Mecak A, Cao C, Dever TE, Burley SK, Cell. 2000 Nov 22;103(5):781-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11114334 11114334]
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/IF2P_METTH IF2P_METTH] Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 (By similarity).
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g7/1g7s_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g7s ConSurf].
+<div style="clear:both"></div>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Methanothermobacter thermautotrophicus]]
-[[Category: Single protein]]
+[[Category: Burley SK]]
-[[Category: Burley, S.K.]]
+[[Category: Cao C]]
-[[Category: Cao, C.]]
+[[Category: Dever TE]]
-[[Category: Dever, T.E.]]
+[[Category: Roll-Mecak A]]
-[[Category: Roll-Mecak, A.]]
-[[Category: GDP]]
-[[Category: translational gtpase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:47:35 2007''