1g5c: Difference between revisions

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-[[Image:1g5c.jpg|left|200px]]<br /><applet load="1g5c" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1g5c, resolution 2.1&Aring;" />
-'''CRYSTAL STRUCTURE OF THE 'CAB' TYPE BETA CLASS CARBONIC ANHYDRASE FROM METHANOBACTERIUM THERMOAUTOTROPHICUM'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF THE 'CAB' TYPE BETA CLASS CARBONIC ANHYDRASE FROM METHANOBACTERIUM THERMOAUTOTROPHICUM==
-The structure of the "cab"-type beta class carbonic anhydrase from the, archaeon Methanobacterium thermoautotrophicum (Cab) has been determined to, 2.1-A resolution using the multiwavelength anomalous diffraction phasing, technique. Cab exists as a dimer with a subunit fold similar to that, observed in "plant"-type beta class carbonic anhydrases. The active site, zinc is coordinated by protein ligands Cys(32), His(87), and Cys(90), with, the tetrahedral coordination completed by a water molecule. The major, difference between plant- and cab-type beta class carbonic anhydrases is, in the organization of the hydrophobic pocket. The structure reveals a, Hepes buffer molecule bound 8 A away from the active site zinc, which, suggests a possible proton transfer pathway from the active site to the, solvent.
+<StructureSection load='1g5c' size='340' side='right'caption='[[1g5c]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1g5c]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G5C FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5c OCA], [https://pdbe.org/1g5c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g5c RCSB], [https://www.ebi.ac.uk/pdbsum/1g5c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g5c ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q50565_METTH Q50565_METTH]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g5/1g5c_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g5c ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-G5C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus] with ZN, CA and EPE as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Carbonate_dehydratase Carbonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G5C OCA].
+*[[Carbonic anhydrase 3D structures|Carbonic anhydrase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Crystal structure of the "cab"-type beta class carbonic anhydrase from the archaeon Methanobacterium thermoautotrophicum., Strop P, Smith KS, Iverson TM, Ferry JG, Rees DC, J Biol Chem. 2001 Mar 30;276(13):10299-305. Epub 2000 Nov 28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11096105 11096105]
+[[Category: Large Structures]]
-[[Category: Carbonate dehydratase]]
 [[Category: Methanothermobacter thermautotrophicus]]
-[[Category: Single protein]]
+[[Category: Ferry JG]]
-[[Category: Ferry, J.G.]]
+[[Category: Iverson TM]]
-[[Category: Iverson, T.M.]]
+[[Category: Rees DC]]
-[[Category: Rees, D.C.]]
+[[Category: Smith KS]]
-[[Category: Smith, K.S.]]
+[[Category: Strop P]]
-[[Category: Strop, P.]]
-[[Category: CA]]
-[[Category: EPE]]
-[[Category: ZN]]
-[[Category: beta carbonic anhydrase]]
-[[Category: hepes]]
-[[Category: zinc]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:51:32 2007''