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| [[Image:1g5b.gif|left|200px]]
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| <!-- | | ==BACTERIOPHAGE LAMBDA SER/THR PROTEIN PHOSPHATASE== |
| The line below this paragraph, containing "STRUCTURE_1g5b", creates the "Structure Box" on the page.
| | <StructureSection load='1g5b' size='340' side='right'caption='[[1g5b]], [[Resolution|resolution]] 2.15Å' scene=''> |
| You may change the PDB parameter (which sets the PDB file loaded into the applet)
| | == Structural highlights == |
| or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| | <table><tr><td colspan='2'>[[1g5b]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G5B FirstGlance]. <br> |
| or leave the SCENE parameter empty for the default display.
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15Å</td></tr> |
| --> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| {{STRUCTURE_1g5b| PDB=1g5b | SCENE= }}
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5b OCA], [https://pdbe.org/1g5b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g5b RCSB], [https://www.ebi.ac.uk/pdbsum/1g5b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g5b ProSAT]</span></td></tr> |
| | </table> |
| | == Function == |
| | [https://www.uniprot.org/uniprot/PP_LAMBD PP_LAMBD] |
| | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> |
| | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g5/1g5b_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> |
| | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g5b ConSurf]. |
| | <div style="clear:both"></div> |
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| '''BACTERIOPHAGE LAMBDA SER/THR PROTEIN PHOSPHATASE'''
| | ==See Also== |
| | | *[[Protein phosphatase 3D structures|Protein phosphatase 3D structures]] |
| | | __TOC__ |
| ==Overview== | | </StructureSection> |
| The protein phosphatase encoded by bacteriophage lambda (lambda PP) belongs to a family of Ser/Thr phosphatases (Ser/Thr PPases) that includes the eukaryotic protein phosphatases 1 (PP1), 2A (PP2A), and 2B (calcineurin). These Ser/Thr PPases and the related purple acid phosphatases (PAPs) contain a conserved phosphoesterase sequence motif that binds a dinuclear metal center. The mechanisms of phosphoester hydrolysis by these enzymes are beginning to be unraveled. To utilize lambda PP more effectively as a model for probing the catalytic mechanism of the Ser/Thr PPases, we have determined its crystal structure to 2.15 A resolution. The overall fold resembles that of PP1 and calcineurin, including a conserved beta alpha beta alpha beta structure that comprises the phosphoesterase motif. Substrates and inhibitors probably bind in a narrow surface groove that houses the active site dinuclear Mn(II) center. The arrangement of metal ligands is similar to that in PP1, calcineurin, and PAP, and a bound sulfate ion is present in two novel coordination modes. In two of the three molecules in the crystallographic asymmetric unit, sulfate is coordinated to Mn2 in a monodentate, terminal fashion, and the two Mn(II) ions are bridged by a solvent molecule. Two additional solvent molecules are coordinated to Mn1. In the third molecule, the sulfate ion is triply coordinated to the metal center with one oxygen coordinated to both Mn(II) ions, one oxygen coordinated to Mn1, and one oxygen coordinated to Mn2. The sulfate in this coordination mode displaces the bridging ligand and one of the terminal solvent ligands. In both sulfate coordination modes, the sulfate ion is stabilized by hydrogen bonding interactions with conserved arginine residues, Arg 53 and Arg 162. The two different active site structures provide models for intermediates in phosphoester hydrolysis and suggest specific mechanistic roles for conserved residues.
| | [[Category: Escherichia virus Lambda]] |
| | | [[Category: Large Structures]] |
| ==About this Structure==
| | [[Category: Reiter NJ]] |
| 1G5B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5B OCA].
| | [[Category: Rosenzweig AC]] |
| | | [[Category: Rusnak F]] |
| ==Reference==
| | [[Category: Voegtli WC]] |
| Structure of the bacteriophage lambda Ser/Thr protein phosphatase with sulfate ion bound in two coordination modes., Voegtli WC, White DJ, Reiter NJ, Rusnak F, Rosenzweig AC, Biochemistry. 2000 Dec 19;39(50):15365-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11112522 11112522]
| | [[Category: White DJ]] |
| [[Category: Enterobacteria phage lambda]] | |
| [[Category: Single protein]] | |
| [[Category: Reiter, N J.]] | |
| [[Category: Rosenzweig, A C.]] | |
| [[Category: Rusnak, F.]] | |
| [[Category: Voegtli, W C.]] | |
| [[Category: White, D J.]] | |
| [[Category: Bacteriophage lambda]]
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| [[Category: Manganese]]
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| [[Category: Ppase]]
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| [[Category: Protein phosphatase]]
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| [[Category: Ser/thr protein phosphatase]]
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| [[Category: Sulfate]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:09:17 2008''
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