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[[Image:1g5b.gif|left|200px]]<br /><applet load="1g5b" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1g5b, resolution 2.15&Aring;" />
'''BACTERIOPHAGE LAMBDA SER/THR PROTEIN PHOSPHATASE'''<br />


==Overview==
==BACTERIOPHAGE LAMBDA SER/THR PROTEIN PHOSPHATASE==
The protein phosphatase encoded by bacteriophage lambda (lambda PP) belongs to a family of Ser/Thr phosphatases (Ser/Thr PPases) that includes the eukaryotic protein phosphatases 1 (PP1), 2A (PP2A), and 2B (calcineurin). These Ser/Thr PPases and the related purple acid phosphatases (PAPs) contain a conserved phosphoesterase sequence motif that binds a dinuclear metal center. The mechanisms of phosphoester hydrolysis by these enzymes are beginning to be unraveled. To utilize lambda PP more effectively as a model for probing the catalytic mechanism of the Ser/Thr PPases, we have determined its crystal structure to 2.15 A resolution. The overall fold resembles that of PP1 and calcineurin, including a conserved beta alpha beta alpha beta structure that comprises the phosphoesterase motif. Substrates and inhibitors probably bind in a narrow surface groove that houses the active site dinuclear Mn(II) center. The arrangement of metal ligands is similar to that in PP1, calcineurin, and PAP, and a bound sulfate ion is present in two novel coordination modes. In two of the three molecules in the crystallographic asymmetric unit, sulfate is coordinated to Mn2 in a monodentate, terminal fashion, and the two Mn(II) ions are bridged by a solvent molecule. Two additional solvent molecules are coordinated to Mn1. In the third molecule, the sulfate ion is triply coordinated to the metal center with one oxygen coordinated to both Mn(II) ions, one oxygen coordinated to Mn1, and one oxygen coordinated to Mn2. The sulfate in this coordination mode displaces the bridging ligand and one of the terminal solvent ligands. In both sulfate coordination modes, the sulfate ion is stabilized by hydrogen bonding interactions with conserved arginine residues, Arg 53 and Arg 162. The two different active site structures provide models for intermediates in phosphoester hydrolysis and suggest specific mechanistic roles for conserved residues.
<StructureSection load='1g5b' size='340' side='right'caption='[[1g5b]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1g5b]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G5B FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5b OCA], [https://pdbe.org/1g5b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g5b RCSB], [https://www.ebi.ac.uk/pdbsum/1g5b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g5b ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PP_LAMBD PP_LAMBD]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g5/1g5b_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g5b ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1G5B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=HG:'>HG</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5B OCA].
*[[Protein phosphatase 3D structures|Protein phosphatase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structure of the bacteriophage lambda Ser/Thr protein phosphatase with sulfate ion bound in two coordination modes., Voegtli WC, White DJ, Reiter NJ, Rusnak F, Rosenzweig AC, Biochemistry. 2000 Dec 19;39(50):15365-74. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11112522 11112522]
[[Category: Escherichia virus Lambda]]
[[Category: Enterobacteria phage lambda]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Reiter NJ]]
[[Category: Reiter, N J.]]
[[Category: Rosenzweig AC]]
[[Category: Rosenzweig, A C.]]
[[Category: Rusnak F]]
[[Category: Rusnak, F.]]
[[Category: Voegtli WC]]
[[Category: Voegtli, W C.]]
[[Category: White DJ]]
[[Category: White, D J.]]
[[Category: HG]]
[[Category: MN]]
[[Category: SO4]]
[[Category: bacteriophage lambda]]
[[Category: manganese]]
[[Category: ppase]]
[[Category: protein phosphatase]]
[[Category: ser/thr protein phosphatase]]
[[Category: sulfate]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:46:15 2008''

Latest revision as of 10:23, 7 February 2024

BACTERIOPHAGE LAMBDA SER/THR PROTEIN PHOSPHATASEBACTERIOPHAGE LAMBDA SER/THR PROTEIN PHOSPHATASE

Structural highlights

1g5b is a 3 chain structure with sequence from Escherichia virus Lambda. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.15Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PP_LAMBD

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1g5b, resolution 2.15Å

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