1g28: Difference between revisions

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New page: left|200px<br /><applet load="1g28" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g28, resolution 2.73Å" /> '''STRUCTURE OF A FLAVI...
 
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[[Image:1g28.gif|left|200px]]<br /><applet load="1g28" size="450" color="white" frame="true" align="right" spinBox="true"
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'''STRUCTURE OF A FLAVIN-BINDING DOMAIN, LOV2, FROM THE CHIMERIC PHYTOCHROME/PHOTOTROPIN PHOTORECEPTOR PHY3'''<br />


==Overview==
==STRUCTURE OF A FLAVIN-BINDING DOMAIN, LOV2, FROM THE CHIMERIC PHYTOCHROME/PHOTOTROPIN PHOTORECEPTOR PHY3==
Phototropin, a major blue-light receptor for phototropism in seed plants, exhibits blue-light-dependent autophosphorylation and contains two light, oxygen, or voltage (LOV) domains and a serine/threonine kinase domain. The, LOV domains share homology with the PER-ARNT-SIM (PAS) superfamily, a, diverse group of sensor proteins. Each LOV domain noncovalently binds a, single FMN molecule and exhibits reversible photochemistry in vitro when, expressed separately or in tandem. We have determined the crystal, structure of the LOV2 domain from the phototropin segment of the chimeric, fern photoreceptor phy3 to 2.7-A resolution. The structure constitutes an, FMN-binding fold that reveals how the flavin cofactor is embedded in the, protein. The single LOV2 cysteine residue is located 4.2 A from flavin, atom C(4a), consistent with a model in which absorption of blue light, induces formation of a covalent cysteinyl-C(4a) adduct. Residues that, interact with FMN in the phototropin segment of the chimeric fern, photoreceptor (phy3) LOV2 are conserved in LOV domains from phototropin of, other plant species and from three proteins involved in the regulation of, circadian rhythms in Arabidopsis and Neurospora. This conservation, suggests that these domains exhibit the same overall fold and share a, common mechanism for flavin binding and light-induced signaling.
<StructureSection load='1g28' size='340' side='right'caption='[[1g28]], [[Resolution|resolution]] 2.73&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1g28]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Adiantum_capillus-veneris Adiantum capillus-veneris]. The March 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Phototropin''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_3 10.2210/rcsb_pdb/mom_2015_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G28 FirstGlance]. <br>
1G28 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Eukaryota Eukaryota] with FMN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G28 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.73&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g28 OCA], [https://pdbe.org/1g28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g28 RCSB], [https://www.ebi.ac.uk/pdbsum/1g28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g28 ProSAT]</span></td></tr>
Structure of a flavin-binding plant photoreceptor domain: insights into light-mediated signal transduction., Crosson S, Moffat K, Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):2995-3000. Epub 2001 Feb 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11248020 11248020]
</table>
[[Category: Eukaryota]]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/Q9ZWQ6_ADICA Q9ZWQ6_ADICA]  
[[Category: Crosson, S.]]
== Evolutionary Conservation ==
[[Category: Moffat, K.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: FMN]]
Check<jmol>
[[Category: alpha-beta structure]]
  <jmolCheckbox>
[[Category: flavoprotein]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g2/1g28_consurf.spt"</scriptWhenChecked>
[[Category: fmn-binding domain]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: lov]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: pas fold]]
  </jmolCheckbox>
[[Category: photoreceptor]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g28 ConSurf].
[[Category: phototropin]]
<div style="clear:both"></div>
 
__TOC__
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:42:32 2007''
</StructureSection>
[[Category: Adiantum capillus-veneris]]
[[Category: Large Structures]]
[[Category: Phototropin]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Crosson S]]
[[Category: Moffat K]]

Latest revision as of 10:21, 7 February 2024

STRUCTURE OF A FLAVIN-BINDING DOMAIN, LOV2, FROM THE CHIMERIC PHYTOCHROME/PHOTOTROPIN PHOTORECEPTOR PHY3STRUCTURE OF A FLAVIN-BINDING DOMAIN, LOV2, FROM THE CHIMERIC PHYTOCHROME/PHOTOTROPIN PHOTORECEPTOR PHY3

Structural highlights

1g28 is a 4 chain structure with sequence from Adiantum capillus-veneris. The March 2015 RCSB PDB Molecule of the Month feature on Phototropin by David Goodsell is 10.2210/rcsb_pdb/mom_2015_3. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.73Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9ZWQ6_ADICA

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1g28, resolution 2.73Å

Drag the structure with the mouse to rotate

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OCA
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