1fts: Difference between revisions

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OCA

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-[[Image:1fts.jpg|left|200px]]<br /><applet load="1fts" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1fts, resolution 2.2&Aring;" />
-'''SIGNAL RECOGNITION PARTICLE RECEPTOR FROM E. COLI'''<br />
-==Overview==
+==SIGNAL RECOGNITION PARTICLE RECEPTOR FROM E. COLI==
-FtsY is the docking protein or SR alpha homologue in E. coli. It is, involved in targeting secretory proteins to the cytoplasmic membrane by, interacting with the signal recognition particle, controlled by guanosine, 5'-triphosphate. Two different constructs have been used in, crystallization studies: the full-length protein and a truncated fragment, with a his-tag at the C terminus. Only the second construct resulted in, crystals suitable for x-ray diffraction. The crystals belong to the, monoclinic space group P2(1) with cell dimensions a = 32.20 A, b = 79.57, A, c = 59.21 A, and beta = 94.45, and contain one molecule per asymmetric, unit. At cryogenic temperatures the crystals diffract to a resolution, limit of 2.5 A by using a rotating anode, and beyond 1.8 A by using, synchrotron radiation.
+<StructureSection load='1fts' size='340' side='right'caption='[[1fts]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1fts]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FTS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FTS FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fts OCA], [https://pdbe.org/1fts PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fts RCSB], [https://www.ebi.ac.uk/pdbsum/1fts PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fts ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/FTSY_ECOLI FTSY_ECOLI] Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC). Interaction with SRP-RNC leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components.<ref>PMID:8194520</ref> <ref>PMID:9305630</ref> <ref>PMID:11735405</ref> <ref>PMID:11741850</ref> <ref>PMID:15148364</ref> <ref>PMID:17682051</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ft/1fts_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fts ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-FTS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FTS OCA].
+*[[Signal recognition particle receptor 3D structures|Signal recognition particle receptor 3D structures]]
+== References ==
-==Reference==
+<references/>
-Expression, crystallization and preliminary X-ray diffraction study of FtsY, the docking protein of the signal recognition particle of E. coli., Montoya G, Svensson C, Luirink J, Sinning I, Proteins. 1997 Jun;28(2):285-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9188744 9188744]
+__TOC__
+</StructureSection>
 [[Category: Escherichia coli]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Luirink, J.]]
+[[Category: Luirink J]]
-[[Category: Montoya, G.]]
+[[Category: Montoya G]]
-[[Category: Sinning, I.]]
+[[Category: Sinning I]]
-[[Category: Svensson, C.]]
+[[Category: Svensson C]]
-[[Category: gtpase]]
-[[Category: protein targeting]]
-[[Category: signal recognition particle receptor]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:16:35 2007''