1fmw: Difference between revisions

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New page: left|200px<br /><applet load="1fmw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fmw, resolution 2.15Å" /> '''CRYSTAL STRUCTURE OF...
 
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'''CRYSTAL STRUCTURE OF THE MGATP COMPLEX FOR THE MOTOR DOMAIN OF DICTYOSTELIUM MYOSIN II'''<br />


==Overview==
==CRYSTAL STRUCTURE OF THE MGATP COMPLEX FOR THE MOTOR DOMAIN OF DICTYOSTELIUM MYOSIN II==
Myosin is the most comprehensively studied molecular motor that converts, energy from the hydrolysis of MgATP into directed movement. Its motile, cycle consists of a sequential series of interactions between myosin, actin, MgATP, and the products of hydrolysis, where the affinity of myosin, for actin is modulated by the nature of the nucleotide bound in the active, site. The first step in the contractile cycle occurs when ATP binds to, actomyosin and releases myosin from the complex. We report here the, structure of the motor domain of Dictyostelium discoideum myosin II both, in its nucleotide-free state and complexed with MgATP. The structure with, MgATP was obtained by soaking the crystals in substrate. These structures, reveal that both the apo form and the MgATP complex are very similar to, those previously seen with MgATPgammaS and MgAMP-PNP. Moreover, these, structures are similar to that of chicken skeletal myosin subfragment-1., The crystallized protein is enzymatically active in solution, indicating, that the conformation of myosin observed in chicken skeletal myosin, subfragment-1 is unable to hydrolyze ATP and most likely represents the, pre-hydrolysis structure for the myosin head that occurs after release, from actin.
<StructureSection load='1fmw' size='340' side='right'caption='[[1fmw]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1fmw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FMW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FMW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fmw OCA], [https://pdbe.org/1fmw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fmw RCSB], [https://www.ebi.ac.uk/pdbsum/1fmw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fmw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fm/1fmw_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fmw ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1FMW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum] with MG and ATP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FMW OCA].
*[[Myosin 3D Structures|Myosin 3D Structures]]
 
__TOC__
==Reference==
</StructureSection>
X-ray structures of the apo and MgATP-bound states of Dictyostelium discoideum myosin motor domain., Bauer CB, Holden HM, Thoden JB, Smith R, Rayment I, J Biol Chem. 2000 Dec 8;275(49):38494-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10954715 10954715]
[[Category: Dictyostelium discoideum]]
[[Category: Dictyostelium discoideum]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bauer, C.B.]]
[[Category: Bauer CB]]
[[Category: Holden, H.M.]]
[[Category: Holden HM]]
[[Category: Rayment, I.]]
[[Category: Rayment I]]
[[Category: Smith, R.]]
[[Category: Smith R]]
[[Category: Thoden, J.B.]]
[[Category: Thoden JB]]
[[Category: ATP]]
[[Category: MG]]
[[Category: myosin motor domaim]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:03:01 2007''

Latest revision as of 10:16, 7 February 2024

CRYSTAL STRUCTURE OF THE MGATP COMPLEX FOR THE MOTOR DOMAIN OF DICTYOSTELIUM MYOSIN IICRYSTAL STRUCTURE OF THE MGATP COMPLEX FOR THE MOTOR DOMAIN OF DICTYOSTELIUM MYOSIN II

Structural highlights

1fmw is a 1 chain structure with sequence from Dictyostelium discoideum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.15Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MYS2_DICDI Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1fmw, resolution 2.15Å

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