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[[Image:1flo.gif|left|200px]]<br /><applet load="1flo" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1flo, resolution 2.65&Aring;" />
'''FLP RECOMBINASE-HOLLIDAY JUNCTION COMPLEX I'''<br />


==Overview==
==FLP Recombinase-Holliday Junction Complex I==
The crystal structure of a Flp recombinase tetramer bound to a Holliday junction intermediate has been determined at 2.65 A resolution. Only one of Flp's two domains, containing the active site, is structurally related to other lambda integrase family site-specific recombinases, such as Cre. The Flp active site differs, however, in that the helix containing the nucleophilic tyrosine is domain swapped, such that it cuts its DNA target in trans. The Flp tetramer displays pseudo four-fold symmetry matching that of the square planar Holliday junction substrate. This tetramer is stabilized by additional novel trans interactions among monomers. The structure illustrates how mechanistic unity is maintained on a chemical level while allowing for substantial variation on the structural level within a family of enzymes.
<StructureSection load='1flo' size='340' side='right'caption='[[1flo]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1flo]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FLO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FLO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PHS:PHOSPHONIC+ACID'>PHS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1flo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1flo OCA], [https://pdbe.org/1flo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1flo RCSB], [https://www.ebi.ac.uk/pdbsum/1flo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1flo ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FLP_YEAST FLP_YEAST] Part of the plasmid amplification system, which corrects any decrease in copy number caused by a rare missegregation event. Catalyzes the recombination between the large inverted repetitions of the 2-micron plasmid during plasmid replication. This recombination event changes the direction of one of the two replication forks in the bidirectionally replicating molecule, effectively resulting in multiple rounds of replication from a single initiation event. Binds specifically to the FLP recognition target (FRT) site where it induces DNA to bend. Three types of bend exist. Type I is approximately 60 degrees and results from 1 FLP molecule binding to 1 symmetry element. Type II is >144 degrees and results from FLP molecules binding to symmetry elements a and b. Type III is approximately 65 degrees and results from FLP molecules binding to symmetry elements b and c.<ref>PMID:2040639</ref> <ref>PMID:3316982</ref> <ref>PMID:2254930</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fl/1flo_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1flo ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1FLO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=PHS:'>PHS</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=CTA:Active+Catalytic+Site+Of+Monomer+A.+Y343+Is+Donated+By+M+...'>CTA</scene>, <scene name='pdbsite=CTB:Active+Catalytic+Site+Of+Monomer+B.+Y343+Is+Donated+By+M+...'>CTB</scene>, <scene name='pdbsite=CTC:Inactive+Catalytic+Site+Of+Monomer+C.+Y343+Is+Donated+By+...'>CTC</scene>, <scene name='pdbsite=CTD:Inactive+Catalytic+Site+Of+Monomer+D.+Y343+Is+Donated+By+...'>CTD</scene> and <scene name='pdbsite=PHO:Scissle+Phosphates'>PHO</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FLO OCA].
*[[Retroviral integrase 3D structures|Retroviral integrase 3D structures]]
 
== References ==
==Reference==
<references/>
Crystal structure of a Flp recombinase-Holliday junction complex: assembly of an active oligomer by helix swapping., Chen Y, Narendra U, Iype LE, Cox MM, Rice PA, Mol Cell. 2000 Oct;6(4):885-97. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11090626 11090626]
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Chen Y]]
[[Category: Chen, Y.]]
[[Category: Cox MM]]
[[Category: Cox, M M.]]
[[Category: Iype LE]]
[[Category: Iype, L E.]]
[[Category: Narendra U]]
[[Category: Narendra, U.]]
[[Category: Rice PA]]
[[Category: Rice, P A.]]
[[Category: PHS]]
[[Category: domain-swapping]]
[[Category: holliday-junction]]
[[Category: protein-dna complex]]
[[Category: tyrosine recombinase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:39:57 2008''

Latest revision as of 10:16, 7 February 2024

FLP Recombinase-Holliday Junction Complex IFLP Recombinase-Holliday Junction Complex I

Structural highlights

1flo is a 12 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.65Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FLP_YEAST Part of the plasmid amplification system, which corrects any decrease in copy number caused by a rare missegregation event. Catalyzes the recombination between the large inverted repetitions of the 2-micron plasmid during plasmid replication. This recombination event changes the direction of one of the two replication forks in the bidirectionally replicating molecule, effectively resulting in multiple rounds of replication from a single initiation event. Binds specifically to the FLP recognition target (FRT) site where it induces DNA to bend. Three types of bend exist. Type I is approximately 60 degrees and results from 1 FLP molecule binding to 1 symmetry element. Type II is >144 degrees and results from FLP molecules binding to symmetry elements a and b. Type III is approximately 65 degrees and results from FLP molecules binding to symmetry elements b and c.[1] [2] [3]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Pan H, Clary D, Sadowski PD. Identification of the DNA-binding domain of the FLP recombinase. J Biol Chem. 1991 Jun 15;266(17):11347-54. PMID:2040639
  2. ↑ Reynolds AE, Murray AW, Szostak JW. Roles of the 2 microns gene products in stable maintenance of the 2 microns plasmid of Saccharomyces cerevisiae. Mol Cell Biol. 1987 Oct;7(10):3566-73. PMID:3316982
  3. ↑ Schwartz CJ, Sadowski PD. FLP protein of 2 mu circle plasmid of yeast induces multiple bends in the FLP recognition target site. J Mol Biol. 1990 Nov 20;216(2):289-98. PMID:2254930

1flo, resolution 2.65Å

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