1fjl: Difference between revisions

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New page: left|200px<br /><applet load="1fjl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fjl, resolution 2.0Å" /> '''HOMEODOMAIN FROM THE ...
 
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[[Image:1fjl.gif|left|200px]]<br /><applet load="1fjl" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1fjl, resolution 2.0&Aring;" />
'''HOMEODOMAIN FROM THE DROSOPHILA PAIRED PROTEIN BOUND TO A DNA OLIGONUCLEOTIDE'''<br />


==Overview==
==HOMEODOMAIN FROM THE DROSOPHILA PAIRED PROTEIN BOUND TO A DNA OLIGONUCLEOTIDE==
The crystal structure of the paired homeodomain bound to DNA as a, cooperative dimer has been determined to 2.0 A resolution. Direct contacts, between each homeodomain and the DNA are similar to those described, previously. In addition, an extensive network of water molecules mediates, contacts between the recognition helix and the DNA major groove. Several, symmetrical contacts between the two homeodomains underlie the cooperative, interaction, and deformations in the DNA structure are necessary for the, establishment of these contacts. Comparison with structures of, homeodomains bound monomerically to DNA suggests that the binding of a, single paired homeodomain can introduce these DNA distortions, thus, preparing a template for the cooperative interaction with a second, homeodomain. This study shows how the paired (Pax) class homeodomains have, achieved cooperativity in DNA binding without the assistance of other, domains, thereby enabling the recognition of target sequences that are, long enough to ensure specificity.
<StructureSection load='1fjl' size='340' side='right'caption='[[1fjl]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1fjl]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FJL FirstGlance]. <br>
1FJL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FJL OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fjl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fjl OCA], [https://pdbe.org/1fjl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fjl RCSB], [https://www.ebi.ac.uk/pdbsum/1fjl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fjl ProSAT]</span></td></tr>
==Reference==
</table>
High resolution crystal structure of a paired (Pax) class cooperative homeodomain dimer on DNA., Wilson DS, Guenther B, Desplan C, Kuriyan J, Cell. 1995 Sep 8;82(5):709-19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7671301 7671301]
== Function ==
[https://www.uniprot.org/uniprot/PRD_DROME PRD_DROME] Pair-rule protein expressed in a segmentally repeating pattern to define the polarity of embryonic segments. Capable of sequence-specific DNA-binding.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fj/1fjl_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fjl ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Desplan, C.]]
[[Category: Desplan C]]
[[Category: Guenther, B.]]
[[Category: Guenther B]]
[[Category: Kuriyan, J.]]
[[Category: Kuriyan J]]
[[Category: Wilson, D.S.]]
[[Category: Wilson DS]]
[[Category: dna]]
[[Category: dna-binding protein]]
[[Category: paired box]]
[[Category: transcription regulation]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:58:42 2007''

Latest revision as of 10:15, 7 February 2024

HOMEODOMAIN FROM THE DROSOPHILA PAIRED PROTEIN BOUND TO A DNA OLIGONUCLEOTIDEHOMEODOMAIN FROM THE DROSOPHILA PAIRED PROTEIN BOUND TO A DNA OLIGONUCLEOTIDE

Structural highlights

1fjl is a 6 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PRD_DROME Pair-rule protein expressed in a segmentally repeating pattern to define the polarity of embryonic segments. Capable of sequence-specific DNA-binding.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1fjl, resolution 2.00Å

Drag the structure with the mouse to rotate

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OCA
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