1fhy: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
(10 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1fhy.gif|left|200px]]


<!--
==PSORALEN CROSS-LINKED D(CCGCTAGCGG) FORMS HOLLIDAY JUNCTION==
The line below this paragraph, containing "STRUCTURE_1fhy", creates the "Structure Box" on the page.
<StructureSection load='1fhy' size='340' side='right'caption='[[1fhy]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1fhy]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FHY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FHY FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PSO:4-HYDROXYMETHYL-4,5,8-TRIMETHYLPSORALEN'>PSO</scene></td></tr>
{{STRUCTURE_1fhy|  PDB=1fhy  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fhy OCA], [https://pdbe.org/1fhy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fhy RCSB], [https://www.ebi.ac.uk/pdbsum/1fhy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fhy ProSAT]</span></td></tr>
 
</table>
'''PSORALEN CROSS-LINKED D(CCGCTAGCGG) FORMS HOLLIDAY JUNCTION'''
__TOC__
 
</StructureSection>
 
[[Category: Large Structures]]
==Overview==
[[Category: Alberti M]]
The single-crystal structures are presented for two DNA sequences with the thymine bases covalently cross-linked across the complementary strands by 4'-hydroxymethyl-4,5',8-trimethylpsoralen (HMT). The HMT-adduct of d(CCGCTAGCGG) forms a psoralen-induced Holliday junction, showing for the first time the effect of this important class of chemotheraputics on the structure of the recombination intermediate. In contrast, HMT-d(CCGGTACCGG) forms a sequence-dependent junction. In both structures, the DNA duplex is highly distorted at the thymine base linked to the six-member pyrone ring of the drug. The psoralen cross-link defines the intramolecular interactions of the drug-induced junction, while the sequence-dependent structure is nearly identical to the native Holliday junction of d(CCGGTACCGG) alone. The two structures contrast the effects of drug- and sequence-dependent interactions on the structure of a Holliday junction, suggesting a role for psoralen in the mechanism to initiate repair of psoralen-lesions in mammalian DNA.
[[Category: Eichman BF]]
 
[[Category: Hearst JE]]
==About this Structure==
[[Category: Ho PS]]
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FHY OCA].  
[[Category: Mooers BHM]]
 
==Reference==
The crystal structures of psoralen cross-linked DNAs: drug-dependent formation of Holliday junctions., Eichman BF, Mooers BH, Alberti M, Hearst JE, Ho PS, J Mol Biol. 2001 Apr 20;308(1):15-26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11302703 11302703]
[[Category: Alberti, M.]]
[[Category: Eichman, B F.]]
[[Category: Hearst, J E.]]
[[Category: Ho, P S.]]
[[Category: Mooers, B H.M.]]
[[Category: 4-way junction]]
[[Category: Cross-linked dna]]
[[Category: Dna-drug complex]]
[[Category: Holliday junction]]
[[Category: Psoralen]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:20:48 2008''

Latest revision as of 10:14, 7 February 2024

PSORALEN CROSS-LINKED D(CCGCTAGCGG) FORMS HOLLIDAY JUNCTIONPSORALEN CROSS-LINKED D(CCGCTAGCGG) FORMS HOLLIDAY JUNCTION

Structural highlights

1fhy is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.2Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

1fhy, resolution 2.20Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=1fhy&oldid=4043492"