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==CRYSTAL STRUCTURE OF RIBOSOMAL PROTEIN TL5, ONE OF THE CTC FAMILY PROTEINS, COMPLEXED WITH A FRAGMENT OF 5S RRNA.==
==CRYSTAL STRUCTURE OF RIBOSOMAL PROTEIN TL5, ONE OF THE CTC FAMILY PROTEINS, COMPLEXED WITH A FRAGMENT OF 5S RRNA.==
<StructureSection load='1feu' size='340' side='right' caption='[[1feu]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
<StructureSection load='1feu' size='340' side='right'caption='[[1feu]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1feu]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"flavobacterium_thermophilum"_yoshida_and_oshima_1971 "flavobacterium thermophilum" yoshida and oshima 1971]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FEU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FEU FirstGlance]. <br>
<table><tr><td colspan='2'>[[1feu]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FEU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FEU FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1feu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1feu OCA], [http://pdbe.org/1feu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1feu RCSB], [http://www.ebi.ac.uk/pdbsum/1feu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1feu ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1feu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1feu OCA], [https://pdbe.org/1feu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1feu RCSB], [https://www.ebi.ac.uk/pdbsum/1feu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1feu ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/RL25_THETH RL25_THETH]] This is one of 3 proteins that mediate the attachment of the 5S rRNA onto the large ribosomal subunit.<ref>PMID:10356982</ref>
[https://www.uniprot.org/uniprot/RL25_THETH RL25_THETH] This is one of 3 proteins that mediate the attachment of the 5S rRNA onto the large ribosomal subunit.<ref>PMID:10356982</ref>  
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1feu ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1feu ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of Thermus thermophilus ribosomal protein TL5 in complex with a fragment of Escherichia coli 5S rRNA has been determined at 2.3 A resolution. The protein consists of two domains. The structure of the N-terminal domain is close to the structure of E. coli ribosomal protein L25, but the C-terminal domain represents a new fold composed of seven beta-strands connected by long loops. TL5 binds to the RNA through its N-terminal domain, whereas the C-terminal domain is not included in this interaction. Cd(2+) ions, the presence of which improved the crystal quality significantly, bind only to the protein component of the complex and stabilize the protein molecule itself and the interactions between the two molecules in the asymmetric unit of the crystal. The TL5 sequence reveals homology to the so-called general stress protein CTC. The hydrophobic cores which stabilize both TL5 domains are highly conserved in CTC proteins. Thus, all CTC proteins may fold with a topology close to that of TL5.


Structure of ribosomal protein TL5 complexed with RNA provides new insights into the CTC family of stress proteins.,Fedorov R, Meshcheryakov V, Gongadze G, Fomenkova N, Nevskaya N, Selmer M, Laurberg M, Kristensen O, Al-Karadaghi S, Liljas A, Garber M, Nikonov S Acta Crystallogr D Biol Crystallogr. 2001 Jul;57(Pt 7):968-76. Epub 2001, Jun 21. PMID:11418764<ref>PMID:11418764</ref>
==See Also==
 
*[[Ribosomal protein L25|Ribosomal protein L25]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1feu" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Flavobacterium thermophilum yoshida and oshima 1971]]
[[Category: Large Structures]]
[[Category: Al-Karadaghi, S]]
[[Category: Thermus thermophilus]]
[[Category: Fedorov, R V]]
[[Category: Al-Karadaghi S]]
[[Category: Fomenkova, N P]]
[[Category: Fedorov RV]]
[[Category: Garber, M B]]
[[Category: Fomenkova NP]]
[[Category: Gongadze, G M]]
[[Category: Garber MB]]
[[Category: Kristensen, O]]
[[Category: Gongadze GM]]
[[Category: Laurberg, M]]
[[Category: Kristensen O]]
[[Category: Liljas, A]]
[[Category: Laurberg M]]
[[Category: Meshcheryakov, V A]]
[[Category: Liljas A]]
[[Category: Nevskaya, N A]]
[[Category: Meshcheryakov VA]]
[[Category: Nikonov, S V]]
[[Category: Nevskaya NA]]
[[Category: Selmer, M]]
[[Category: Nikonov SV]]
[[Category: 5s rrna-protein complex]]
[[Category: Selmer M]]
[[Category: Cadmium ion]]
[[Category: General stress protein ctc]]
[[Category: Ribosome]]

Latest revision as of 10:13, 7 February 2024

CRYSTAL STRUCTURE OF RIBOSOMAL PROTEIN TL5, ONE OF THE CTC FAMILY PROTEINS, COMPLEXED WITH A FRAGMENT OF 5S RRNA.CRYSTAL STRUCTURE OF RIBOSOMAL PROTEIN TL5, ONE OF THE CTC FAMILY PROTEINS, COMPLEXED WITH A FRAGMENT OF 5S RRNA.

Structural highlights

1feu is a 6 chain structure with sequence from Thermus thermophilus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.3Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RL25_THETH This is one of 3 proteins that mediate the attachment of the 5S rRNA onto the large ribosomal subunit.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Gongadze GM, Meshcheryakov VA, Serganov AA, Fomenkova NP, Mudrik ES, Jonsson BH, Liljas A, Nikonov SV, Garber MB. N-terminal domain, residues 1-91, of ribosomal protein TL5 from Thermus thermophilus binds specifically and strongly to the region of 5S rRNA containing loop E. FEBS Lett. 1999 May 14;451(1):51-5. PMID:10356982

1feu, resolution 2.30Å

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