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[[Image:1fcw.gif|left|200px]]


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==TRNA POSITIONS DURING THE ELONGATION CYCLE==
The line below this paragraph, containing "STRUCTURE_1fcw", creates the "Structure Box" on the page.
<SX load='1fcw' size='340' side='right' viewer='molstar' caption='[[1fcw]], [[Resolution|resolution]] 17.00&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1fcw]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FCW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FCW FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 17&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1MA:6-HYDRO-1-METHYLADENOSINE-5-MONOPHOSPHATE'>1MA</scene>, <scene name='pdbligand=2MG:2N-METHYLGUANOSINE-5-MONOPHOSPHATE'>2MG</scene>, <scene name='pdbligand=5MC:5-METHYLCYTIDINE-5-MONOPHOSPHATE'>5MC</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=M2G:N2-DIMETHYLGUANOSINE-5-MONOPHOSPHATE'>M2G</scene>, <scene name='pdbligand=OMC:O2-METHYLYCYTIDINE-5-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=OMG:O2-METHYLGUANOSINE-5-MONOPHOSPHATE'>OMG</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene>, <scene name='pdbligand=YG:WYBUTOSINE'>YG</scene></td></tr>
{{STRUCTURE_1fcw|  PDB=1fcw |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fcw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fcw OCA], [https://pdbe.org/1fcw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fcw RCSB], [https://www.ebi.ac.uk/pdbsum/1fcw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fcw ProSAT]</span></td></tr>
</table>


'''TRNA POSITIONS DURING THE ELONGATION CYCLE'''
==See Also==
 
*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
 
__TOC__
==Overview==
</SX>
Three-dimensional cryomaps have been reconstructed for tRNA-ribosome complexes in pre- and posttranslocational states at 17-A resolution. The positions of tRNAs in the A and P sites in the pretranslocational complexes and in the P and E sites in the posttranslocational complexes have been determined. Of these, the P-site tRNA position is the same as seen earlier in the initiation-like fMet-tRNA(f)(Met)-ribosome complex, where it was visualized with high accuracy. Now, the positions of the A- and E-site tRNAs are determined with similar accuracy. The positions of the CCA end of the tRNAs at the A site are different before and after peptide bond formation. The relative positions of anticodons of P- and E-site tRNAs in the posttranslocational state are such that a codon-anticodon interaction at the E site appears feasible.
[[Category: Large Structures]]
 
[[Category: Saccharomyces cerevisiae]]
==About this Structure==
[[Category: Agrawal RK]]
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FCW OCA].
[[Category: Frank J]]
 
[[Category: Grassucci RA]]
==Reference==
[[Category: Nierhaus KH]]
Visualization of tRNA movements on the Escherichia coli 70S ribosome during the elongation cycle., Agrawal RK, Spahn CM, Penczek P, Grassucci RA, Nierhaus KH, Frank J, J Cell Biol. 2000 Aug 7;150(3):447-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10931859 10931859]
[[Category: Penczek P]]
[[Category: Agrawal, R K.]]
[[Category: Spahn CMT]]
[[Category: Frank, J.]]
[[Category: Grassucci, R A.]]
[[Category: Nierhaus, K H.]]
[[Category: Penczek, P.]]
[[Category: Spahn, C M.T.]]
[[Category: Cryo-electron microscopy]]
[[Category: Elongation cycle]]
[[Category: Protein synthesis]]
[[Category: Ribosome]]
[[Category: Trna binding site]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:11:10 2008''

Latest revision as of 10:13, 7 February 2024

TRNA POSITIONS DURING THE ELONGATION CYCLETRNA POSITIONS DURING THE ELONGATION CYCLE

1fcw, resolution 17.00Å

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