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| [[Image:1fcw.gif|left|200px]]
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| {{Structure
| | ==TRNA POSITIONS DURING THE ELONGATION CYCLE== |
| |PDB= 1fcw |SIZE=350|CAPTION= <scene name='initialview01'>1fcw</scene>, resolution 17.0Å
| | <SX load='1fcw' size='340' side='right' viewer='molstar' caption='[[1fcw]], [[Resolution|resolution]] 17.00Å' scene=''> |
| |SITE= | | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=1MA:6-HYDRO-1-METHYLADENOSINE-5'-MONOPHOSPHATE'>1MA</scene>, <scene name='pdbligand=2MG:2N-METHYLGUANOSINE-5'-MONOPHOSPHATE'>2MG</scene>, <scene name='pdbligand=5MC:5-METHYLCYTIDINE-5'-MONOPHOSPHATE'>5MC</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5'-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5'-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5'-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=M2G:N2-DIMETHYLGUANOSINE-5'-MONOPHOSPHATE'>M2G</scene>, <scene name='pdbligand=OMC:O2'-METHYLYCYTIDINE-5'-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=OMG:O2'-METHYLGUANOSINE-5'-MONOPHOSPHATE'>OMG</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5'-MONOPHOSPHATE'>PSU</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>, <scene name='pdbligand=YG:WYBUTOSINE'>YG</scene>
| | <table><tr><td colspan='2'>[[1fcw]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FCW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FCW FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 17Å</td></tr> |
| |GENE=
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1MA:6-HYDRO-1-METHYLADENOSINE-5-MONOPHOSPHATE'>1MA</scene>, <scene name='pdbligand=2MG:2N-METHYLGUANOSINE-5-MONOPHOSPHATE'>2MG</scene>, <scene name='pdbligand=5MC:5-METHYLCYTIDINE-5-MONOPHOSPHATE'>5MC</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=M2G:N2-DIMETHYLGUANOSINE-5-MONOPHOSPHATE'>M2G</scene>, <scene name='pdbligand=OMC:O2-METHYLYCYTIDINE-5-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=OMG:O2-METHYLGUANOSINE-5-MONOPHOSPHATE'>OMG</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene>, <scene name='pdbligand=YG:WYBUTOSINE'>YG</scene></td></tr> |
| |DOMAIN=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fcw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fcw OCA], [https://pdbe.org/1fcw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fcw RCSB], [https://www.ebi.ac.uk/pdbsum/1fcw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fcw ProSAT]</span></td></tr> |
| |RELATEDENTRY=[[trna09|TRNA09]]
| | </table> |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fcw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fcw OCA], [http://www.ebi.ac.uk/pdbsum/1fcw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fcw RCSB]</span>
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| }}
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| '''TRNA POSITIONS DURING THE ELONGATION CYCLE'''
| | ==See Also== |
| | | *[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]] |
| | | __TOC__ |
| ==Overview== | | </SX> |
| Three-dimensional cryomaps have been reconstructed for tRNA-ribosome complexes in pre- and posttranslocational states at 17-A resolution. The positions of tRNAs in the A and P sites in the pretranslocational complexes and in the P and E sites in the posttranslocational complexes have been determined. Of these, the P-site tRNA position is the same as seen earlier in the initiation-like fMet-tRNA(f)(Met)-ribosome complex, where it was visualized with high accuracy. Now, the positions of the A- and E-site tRNAs are determined with similar accuracy. The positions of the CCA end of the tRNAs at the A site are different before and after peptide bond formation. The relative positions of anticodons of P- and E-site tRNAs in the posttranslocational state are such that a codon-anticodon interaction at the E site appears feasible.
| | [[Category: Large Structures]] |
| | |
| ==About this Structure==
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| 1FCW is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FCW OCA].
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| ==Reference==
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| Visualization of tRNA movements on the Escherichia coli 70S ribosome during the elongation cycle., Agrawal RK, Spahn CM, Penczek P, Grassucci RA, Nierhaus KH, Frank J, J Cell Biol. 2000 Aug 7;150(3):447-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10931859 10931859]
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| [[Category: Protein complex]] | |
| [[Category: Saccharomyces cerevisiae]] | | [[Category: Saccharomyces cerevisiae]] |
| [[Category: Agrawal, R K.]] | | [[Category: Agrawal RK]] |
| [[Category: Frank, J.]] | | [[Category: Frank J]] |
| [[Category: Grassucci, R A.]] | | [[Category: Grassucci RA]] |
| [[Category: Nierhaus, K H.]] | | [[Category: Nierhaus KH]] |
| [[Category: Penczek, P.]] | | [[Category: Penczek P]] |
| [[Category: Spahn, C M.T.]] | | [[Category: Spahn CMT]] |
| [[Category: cryo-electron microscopy]]
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| [[Category: elongation cycle]]
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| [[Category: protein synthesis]]
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| [[Category: ribosome]]
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| [[Category: trna binding site]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:20:29 2008''
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