1fca: Difference between revisions

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New page: left|200px<br /><applet load="1fca" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fca, resolution 1.8Å" /> '''STRUCTURE OF THE FERR...
 
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[[Image:1fca.jpg|left|200px]]<br /><applet load="1fca" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1fca, resolution 1.8&Aring;" />
'''STRUCTURE OF THE FERREDOXIN FROM CLOSTRIDIUM ACIDURICI: MODEL AT 1.8 ANGSTROMS RESOLUTION'''<br />


==Overview==
==STRUCTURE OF THE FERREDOXIN FROM CLOSTRIDIUM ACIDURICI: MODEL AT 1.8 ANGSTROMS RESOLUTION==
Ferredoxins (Fd) are electron-carrier proteins, the active sites of which, are organized around clusters made of iron and inorganic sulfur. The Fd, from Clostridium acidurici is 55 amino acids long and contains two, [4Fe-4S] clusters. Crystals have been obtained in the space group, P4(3)2(1)2, a = b = 34.441 (5), c = 74.778 (9) A. The structure was solved, by molecular replacement using the Fd from Peptostreptcoccus, asaccharolyticus as a search model, these two ferredoxins having 37, residues in common. Refinement using molecular-dynamics techniques was, then initiated. Successive rounds of model building and refinement gave a, structure that includes 45 water molecules with R = 15%. At this stage, the electron-density map clearly revealed discrepancies in the position of, two amino acids in the published primary sequence. Refinement based on, these modifications led to R = 14.3% for 3921 reflections up to 1.8 A, resolution. The geometry of the two clusters has been found to be in good, agreement with that previously obtained at a lower resolution., Interactions of polypeptide chain with the [4Fe-4S] clusters, the cluster, geometry as well as the hydrogen bonds involving S, Sgamma, N and water, molecules are reported.
<StructureSection load='1fca' size='340' side='right'caption='[[1fca]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1fca]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gottschalkia_acidurici Gottschalkia acidurici]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FCA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fca OCA], [https://pdbe.org/1fca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fca RCSB], [https://www.ebi.ac.uk/pdbsum/1fca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fca ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FER_GOTA9 FER_GOTA9] Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.[UniProtKB:P50727]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fc/1fca_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fca ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1FCA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_acidurici Clostridium acidurici] with SF4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FCA OCA].
*[[Ferredoxin 3D structures|Ferredoxin 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structure of the ferredoxin from Clostridium acidurici: model at 1.8 A resolution., TranQui D, Jesior JC, Acta Crystallogr D Biol Crystallogr. 1995 Mar 1;51(Pt 2):155-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299316 15299316]
[[Category: Gottschalkia acidurici]]
[[Category: Clostridium acidurici]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Jesior JC]]
[[Category: Jesior, J.C.]]
[[Category: Tranqui D]]
[[Category: Tranqui, D.]]
[[Category: SF4]]
[[Category: electron transport (cytochrome)]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:48:45 2007''

Latest revision as of 10:13, 7 February 2024

STRUCTURE OF THE FERREDOXIN FROM CLOSTRIDIUM ACIDURICI: MODEL AT 1.8 ANGSTROMS RESOLUTIONSTRUCTURE OF THE FERREDOXIN FROM CLOSTRIDIUM ACIDURICI: MODEL AT 1.8 ANGSTROMS RESOLUTION

Structural highlights

1fca is a 1 chain structure with sequence from Gottschalkia acidurici. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.8Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FER_GOTA9 Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.[UniProtKB:P50727]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1fca, resolution 1.80Å

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