1f7s: Difference between revisions

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-[[Image:1f7s.jpg|left|200px]]
-<!--
+==CRYSTAL STRUCTURE OF ADF1 FROM ARABIDOPSIS THALIANA==
-The line below this paragraph, containing "STRUCTURE_1f7s", creates the "Structure Box" on the page.
+<StructureSection load='1f7s' size='340' side='right'caption='[[1f7s]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1f7s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F7S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F7S FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene></td></tr>
-{{STRUCTURE_1f7s|  PDB=1f7s  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f7s OCA], [https://pdbe.org/1f7s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f7s RCSB], [https://www.ebi.ac.uk/pdbsum/1f7s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f7s ProSAT]</span></td></tr>
+</table>
-'''CRYSTAL STRUCTURE OF ADF1 FROM ARABIDOPSIS THALIANA'''
+== Function ==
+[https://www.uniprot.org/uniprot/ADF1_ARATH ADF1_ARATH] Actin-depolymerizing protein. Stimulates F-actin depolymerization. Involved in plant development, cell organ expansion and flowering by controlling breakdown of thick actin cables.<ref>PMID:11402164</ref>
+== Evolutionary Conservation ==
-==Overview==
+[[Image:Consurf_key_small.gif|200px|right]]
-Actin-depolymerizing factor (ADF) and cofilin define a family of actin-binding proteins essential for the rapid turnover of filamentous actin in vivo. Here we present the 2.0 A crystal structure of Arabidopsis thaliana ADF1 (AtADF1), the first plant crystal structure from the ADF/cofilin (AC) family. Superposition of the four AC isoform structures permits an accurate sequence alignment that differs from previously reported data for the location of vertebrate-specific inserts and reveals a contiguous, vertebrate-specific surface opposite the putative actin-binding surface. Extending the structure-based sequence alignment to include 30 additional isoforms indicates three major groups: vertebrates, plants, and "other eukaryotes." Within these groups, several structurally conserved residues that are not conserved throughout the entire AC family have been identified. Residues that are highly conserved among all isoforms tend to cluster around the tryptophan at position 90 and a structurally conserved kink in alpha-helix 3. Analysis of surface character shows the presence of a hydrophobic patch and a highly conserved acidic cluster, both of which include several residues previously implicated in actin binding.
+Check<jmol>
+  <jmolCheckbox>
-==About this Structure==
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f7/1f7s_consurf.spt"</scriptWhenChecked>
-F7S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F7S OCA].
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
-==Reference==
+  </jmolCheckbox>
-A comparative structural analysis of the ADF/cofilin family., Bowman GD, Nodelman IM, Hong Y, Chua NH, Lindberg U, Schutt CE, Proteins. 2000 Nov 15;41(3):374-84. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11025548 11025548]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f7s ConSurf].
+<div style="clear:both"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Arabidopsis thaliana]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Bowman, G D.]]
+[[Category: Bowman GD]]
-[[Category: Chua, N H.]]
+[[Category: Chua NH]]
-[[Category: Lindberg, U.]]
+[[Category: Lindberg U]]
-[[Category: Nodelman, I M.]]
+[[Category: Nodelman IM]]
-[[Category: Schutt, C E.]]
+[[Category: Schutt CE]]
-[[Category: Kink in alpha-helix 3]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 16:00:18 2008''