1f7s: Difference between revisions

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[[Image:1f7s.jpg|left|200px]]


{{Structure
==CRYSTAL STRUCTURE OF ADF1 FROM ARABIDOPSIS THALIANA==
|PDB= 1f7s |SIZE=350|CAPTION= <scene name='initialview01'>1f7s</scene>, resolution 2.0&Aring;
<StructureSection load='1f7s' size='340' side='right'caption='[[1f7s]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=LDA:LAURYL DIMETHYLAMINE-N-OXIDE'>LDA</scene>
<table><tr><td colspan='2'>[[1f7s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F7S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F7S FirstGlance]. <br>
|ACTIVITY=
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f7s OCA], [https://pdbe.org/1f7s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f7s RCSB], [https://www.ebi.ac.uk/pdbsum/1f7s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f7s ProSAT]</span></td></tr>
 
</table>
'''CRYSTAL STRUCTURE OF ADF1 FROM ARABIDOPSIS THALIANA'''
== Function ==
 
[https://www.uniprot.org/uniprot/ADF1_ARATH ADF1_ARATH] Actin-depolymerizing protein. Stimulates F-actin depolymerization. Involved in plant development, cell organ expansion and flowering by controlling breakdown of thick actin cables.<ref>PMID:11402164</ref>
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
Actin-depolymerizing factor (ADF) and cofilin define a family of actin-binding proteins essential for the rapid turnover of filamentous actin in vivo. Here we present the 2.0 A crystal structure of Arabidopsis thaliana ADF1 (AtADF1), the first plant crystal structure from the ADF/cofilin (AC) family. Superposition of the four AC isoform structures permits an accurate sequence alignment that differs from previously reported data for the location of vertebrate-specific inserts and reveals a contiguous, vertebrate-specific surface opposite the putative actin-binding surface. Extending the structure-based sequence alignment to include 30 additional isoforms indicates three major groups: vertebrates, plants, and "other eukaryotes." Within these groups, several structurally conserved residues that are not conserved throughout the entire AC family have been identified. Residues that are highly conserved among all isoforms tend to cluster around the tryptophan at position 90 and a structurally conserved kink in alpha-helix 3. Analysis of surface character shows the presence of a hydrophobic patch and a highly conserved acidic cluster, both of which include several residues previously implicated in actin binding.
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f7/1f7s_consurf.spt"</scriptWhenChecked>
1F7S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F7S OCA].  
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Reference==
  </jmolCheckbox>
A comparative structural analysis of the ADF/cofilin family., Bowman GD, Nodelman IM, Hong Y, Chua NH, Lindberg U, Schutt CE, Proteins. 2000 Nov 15;41(3):374-84. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11025548 11025548]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f7s ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bowman, G D.]]
[[Category: Bowman GD]]
[[Category: Chua, N H.]]
[[Category: Chua NH]]
[[Category: Lindberg, U.]]
[[Category: Lindberg U]]
[[Category: Nodelman, I M.]]
[[Category: Nodelman IM]]
[[Category: Schutt, C E.]]
[[Category: Schutt CE]]
[[Category: LDA]]
[[Category: kink in alpha-helix 3]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:05:36 2008''

Latest revision as of 10:11, 7 February 2024

CRYSTAL STRUCTURE OF ADF1 FROM ARABIDOPSIS THALIANACRYSTAL STRUCTURE OF ADF1 FROM ARABIDOPSIS THALIANA

Structural highlights

1f7s is a 1 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ADF1_ARATH Actin-depolymerizing protein. Stimulates F-actin depolymerization. Involved in plant development, cell organ expansion and flowering by controlling breakdown of thick actin cables.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Dong CH, Xia GX, Hong Y, Ramachandran S, Kost B, Chua NH. ADF proteins are involved in the control of flowering and regulate F-actin organization, cell expansion, and organ growth in Arabidopsis. Plant Cell. 2001 Jun;13(6):1333-46. PMID:11402164

1f7s, resolution 2.00Å

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