1f2t: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
New page: left|200px<br /><applet load="1f2t" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f2t, resolution 1.6Å" /> '''CRYSTAL STRUCTURE OF ...
 
No edit summary
 
(16 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1f2t.gif|left|200px]]<br /><applet load="1f2t" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1f2t, resolution 1.6&Aring;" />
'''CRYSTAL STRUCTURE OF ATP-FREE RAD50 ABC-ATPASE'''<br />


==Overview==
==Crystal Structure of ATP-Free RAD50 ABC-ATPase==
To clarify the key role of Rad50 in DNA double-strand break repair (DSBR), we biochemically and structurally characterized ATP-bound and ATP-free, Rad50 catalytic domain (Rad50cd) from Pyrococcus furiosus. Rad50cd, displays ATPase activity plus ATP-controlled dimerization and DNA binding, activities. Rad50cd crystal structures identify probable protein and DNA, interfaces and reveal an ABC-ATPase fold, linking Rad50 molecular, mechanisms to ABC transporters, including P glycoprotein and cystic, fibrosis transmembrane conductance regulator. Binding of ATP, gamma-phosphates to conserved signature motifs in two opposing Rad50cd, molecules promotes dimerization that likely couples ATP hydrolysis to, dimer dissociation and DNA release. These results, validated by mutations, suggest unified molecular mechanisms for ATP-driven cooperativity and, allosteric control of ABC-ATPases in DSBR, membrane transport, and, chromosome condensation by SMC proteins.
<StructureSection load='1f2t' size='340' side='right'caption='[[1f2t]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1f2t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F2T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F2T FirstGlance]. <br>
1F2T is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F2T OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f2t OCA], [https://pdbe.org/1f2t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f2t RCSB], [https://www.ebi.ac.uk/pdbsum/1f2t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f2t ProSAT]</span></td></tr>
==Reference==
</table>
Structural biology of Rad50 ATPase: ATP-driven conformational control in DNA double-strand break repair and the ABC-ATPase superfamily., Hopfner KP, Karcher A, Shin DS, Craig L, Arthur LM, Carney JP, Tainer JA, Cell. 2000 Jun 23;101(7):789-800. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10892749 10892749]
== Function ==
[[Category: Protein complex]]
[https://www.uniprot.org/uniprot/RAD50_PYRFU RAD50_PYRFU] Involved in DNA double-strand break repair (DSBR). The Rad50/Mre11 complex possesses single-strand endonuclease activity and ATP-dependent double-strand-specific 3'-5' exonuclease activity. Rad50 provides an ATP-dependent control of Mre11 by unwinding and/or repositioning DNA ends into the Mre11 active site.[HAMAP-Rule:MF_00449]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f2/1f2t_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f2t ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pyrococcus furiosus]]
[[Category: Pyrococcus furiosus]]
[[Category: Craig, L.]]
[[Category: Craig L]]
[[Category: Hopfner, K.P.]]
[[Category: Hopfner KP]]
[[Category: Karcher, A.]]
[[Category: Karcher A]]
[[Category: Shin, D.S.]]
[[Category: Shin DS]]
[[Category: abc atpase]]
[[Category: dna double-strand break repair]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:33:29 2007''

Latest revision as of 10:09, 7 February 2024

Crystal Structure of ATP-Free RAD50 ABC-ATPaseCrystal Structure of ATP-Free RAD50 ABC-ATPase

Structural highlights

1f2t is a 2 chain structure with sequence from Pyrococcus furiosus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.6Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RAD50_PYRFU Involved in DNA double-strand break repair (DSBR). The Rad50/Mre11 complex possesses single-strand endonuclease activity and ATP-dependent double-strand-specific 3'-5' exonuclease activity. Rad50 provides an ATP-dependent control of Mre11 by unwinding and/or repositioning DNA ends into the Mre11 active site.[HAMAP-Rule:MF_00449]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1f2t, resolution 1.60Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=1f2t&oldid=4043117"