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| [[Image:1eyu.gif|left|200px]]
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| <!-- | | ==HIGH RESOLUTION STRUCTURE OF THE PVUII ENDONCULEASE/COGNATE DNA COMPLEX AT PH 4.6== |
| The line below this paragraph, containing "STRUCTURE_1eyu", creates the "Structure Box" on the page.
| | <StructureSection load='1eyu' size='340' side='right'caption='[[1eyu]], [[Resolution|resolution]] 1.78Å' scene=''> |
| You may change the PDB parameter (which sets the PDB file loaded into the applet)
| | == Structural highlights == |
| or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| | <table><tr><td colspan='2'>[[1eyu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Proteus_vulgaris Proteus vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EYU FirstGlance]. <br> |
| or leave the SCENE parameter empty for the default display.
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.78Å</td></tr> |
| --> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eyu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eyu OCA], [https://pdbe.org/1eyu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eyu RCSB], [https://www.ebi.ac.uk/pdbsum/1eyu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eyu ProSAT]</span></td></tr> |
| {{STRUCTURE_1eyu| PDB=1eyu | SCENE= }}
| | </table> |
| | == Function == |
| | [https://www.uniprot.org/uniprot/T2P2_PROHU T2P2_PROHU] Recognizes the double-stranded sequence CAGCTG and cleaves after G-3. |
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| '''HIGH RESOLUTION STRUCTURE OF THE PVUII ENDONCULEASE/COGNATE DNA COMPLEX AT PH 4.6'''
| | ==See Also== |
| | | *[[Endonuclease 3D structures|Endonuclease 3D structures]] |
| | | __TOC__ |
| ==Overview==
| | </StructureSection> |
| Restriction endonucleases differ in their use of metal cofactors despite having remarkably similar folds for their catalytic regions. To explore this, we have characterized the interaction of endonuclease PvuII with the catalytically incompetent cation Ca(2+). The structure of a glutaraldehyde-crosslinked crystal of the endonuclease PvuII-DNA complex, determined in the presence of Ca(2+) at a pH of approximately 6.5, supports a two-metal mechanism of DNA cleavage by PvuII. The first Ca(2+) position matches that found in all structurally examined endonucleases, while the second position is similar to that of EcoRV but is distinct from that of BamHI and BglI. The location of the second metal in PvuII, unlike that in BamHI/BglI, permits no direct interaction between the second metal and the O3' oxygen leaving group. However, the interactions between the DNA scissile phosphate and the metals, the first metal and the attacking water, and the attacking water and DNA are the same in PvuII as they are in the two-metal models of BamHI and BglI, but are distinct from the proposed three-metal or the two-metal models of EcoRV.
| | [[Category: Large Structures]] |
| | |
| ==About this Structure== | |
| 1EYU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Proteus_vulgaris Proteus vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYU OCA].
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| ==Reference==
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| PvuII endonuclease contains two calcium ions in active sites., Horton JR, Cheng X, J Mol Biol. 2000 Jul 28;300(5):1049-56. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10903853 10903853]
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| [[Category: Proteus vulgaris]] | | [[Category: Proteus vulgaris]] |
| [[Category: Single protein]]
| | [[Category: Cheng X]] |
| [[Category: Type II site-specific deoxyribonuclease]]
| | [[Category: Horton JR]] |
| [[Category: Cheng, X.]] | |
| [[Category: Horton, J R.]] | |
| [[Category: Endonuclease type ii]]
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| [[Category: Protein-dna complex]]
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| [[Category: Restriction enzyme]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:41:02 2008''
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