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[[Image:1eyg.gif|left|200px]]


{{Structure
==Crystal structure of chymotryptic fragment of E. coli ssb bound to two 35-mer single strand DNAS==
|PDB= 1eyg |SIZE=350|CAPTION= <scene name='initialview01'>1eyg</scene>, resolution 2.80&Aring;
<StructureSection load='1eyg' size='340' side='right'caption='[[1eyg]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>
<table><tr><td colspan='2'>[[1eyg]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EYG FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
|GENE=  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eyg OCA], [https://pdbe.org/1eyg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eyg RCSB], [https://www.ebi.ac.uk/pdbsum/1eyg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eyg ProSAT]</span></td></tr>
|DOMAIN=
</table>
|RELATEDENTRY=[[1kaw|1KAW]]
== Function ==
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eyg OCA], [http://www.ebi.ac.uk/pdbsum/1eyg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1eyg RCSB]</span>
[https://www.uniprot.org/uniprot/SSB_ECOLI SSB_ECOLI] This protein is essential for replication of the chromosomes and its single-stranded DNA phages. It is also involved in DNA recombination and repair.
}}
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ey/1eyg_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eyg ConSurf].
<div style="clear:both"></div>


'''Crystal structure of chymotryptic fragment of E. coli ssb bound to two 35-mer single strand DNAS'''
==See Also==
 
*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
The structure of the homotetrameric DNA binding domain of the single stranded DNA binding protein from Escherichia coli (Eco SSB) bound to two 35-mer single stranded DNAs was determined to a resolution of 2.8 A. This structure describes the vast network of interactions that results in the extensive wrapping of single stranded DNA around the SSB tetramer and suggests a structural basis for its various binding modes.
 
==About this Structure==
1EYG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYG OCA].
 
==Reference==
Structure of the DNA binding domain of E. coli SSB bound to ssDNA., Raghunathan S, Kozlov AG, Lohman TM, Waksman G, Nat Struct Biol. 2000 Aug;7(8):648-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10932248 10932248]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Raghunathan, S.]]
[[Category: Raghunathan S]]
[[Category: Waksman, G.]]
[[Category: Waksman G]]
[[Category: binding mode]]
[[Category: mad phasing]]
[[Category: ob fold]]
[[Category: protein-dna complex]]
[[Category: se-met]]
[[Category: ssb]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:12:08 2008''

Latest revision as of 10:08, 7 February 2024

Crystal structure of chymotryptic fragment of E. coli ssb bound to two 35-mer single strand DNASCrystal structure of chymotryptic fragment of E. coli ssb bound to two 35-mer single strand DNAS

Structural highlights

1eyg is a 6 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.8Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SSB_ECOLI This protein is essential for replication of the chromosomes and its single-stranded DNA phages. It is also involved in DNA recombination and repair.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1eyg, resolution 2.80Å

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