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==Crystal structure of chymotryptic fragment of E. coli ssb bound to two 35-mer single strand DNAS==
==Crystal structure of chymotryptic fragment of E. coli ssb bound to two 35-mer single strand DNAS==
<StructureSection load='1eyg' size='340' side='right' caption='[[1eyg]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
<StructureSection load='1eyg' size='340' side='right'caption='[[1eyg]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1eyg]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EYG FirstGlance]. <br>
<table><tr><td colspan='2'>[[1eyg]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EYG FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1kaw|1kaw]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eyg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1eyg RCSB], [http://www.ebi.ac.uk/pdbsum/1eyg PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eyg OCA], [https://pdbe.org/1eyg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eyg RCSB], [https://www.ebi.ac.uk/pdbsum/1eyg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eyg ProSAT]</span></td></tr>
<table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SSB_ECOLI SSB_ECOLI] This protein is essential for replication of the chromosomes and its single-stranded DNA phages. It is also involved in DNA recombination and repair.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ey/1eyg_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ey/1eyg_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
   </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eyg ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The structure of the homotetrameric DNA binding domain of the single stranded DNA binding protein from Escherichia coli (Eco SSB) bound to two 35-mer single stranded DNAs was determined to a resolution of 2.8 A. This structure describes the vast network of interactions that results in the extensive wrapping of single stranded DNA around the SSB tetramer and suggests a structural basis for its various binding modes.
Structure of the DNA binding domain of E. coli SSB bound to ssDNA.,Raghunathan S, Kozlov AG, Lohman TM, Waksman G Nat Struct Biol. 2000 Aug;7(8):648-52. PMID:10932248<ref>PMID:10932248</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
*[[Single stranded binding protein|Single stranded binding protein]]
*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
*[[Single-stranded DNA-binding protein|Single-stranded DNA-binding protein]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Raghunathan, S.]]
[[Category: Large Structures]]
[[Category: Waksman, G.]]
[[Category: Raghunathan S]]
[[Category: Binding mode]]
[[Category: Waksman G]]
[[Category: Mad phasing]]
[[Category: Ob fold]]
[[Category: Protein-dna complex]]
[[Category: Replication-dna complex]]
[[Category: Se-met]]
[[Category: Ssb]]

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