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| [[Image:1euq.gif|left|200px]]
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| <!-- | | ==CRYSTAL STRUCTURE OF GLUTAMINYL-TRNA SYNTHETASE COMPLEXED WITH A TRNA-GLN MUTANT AND AN ACTIVE-SITE INHIBITOR== |
| The line below this paragraph, containing "STRUCTURE_1euq", creates the "Structure Box" on the page.
| | <StructureSection load='1euq' size='340' side='right'caption='[[1euq]], [[Resolution|resolution]] 3.10Å' scene=''> |
| You may change the PDB parameter (which sets the PDB file loaded into the applet)
| | == Structural highlights == |
| or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| | <table><tr><td colspan='2'>[[1euq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The April 2001 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Aminoacyl-tRNA Synthetases'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2001_4 10.2210/rcsb_pdb/mom_2001_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EUQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EUQ FirstGlance]. <br> |
| or leave the SCENE parameter empty for the default display.
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1Å</td></tr> |
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| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=QSI:5-O-[N-(L-GLUTAMINYL)-SULFAMOYL]ADENOSINE'>QSI</scene></td></tr> |
| {{STRUCTURE_1euq| PDB=1euq | SCENE= }}
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1euq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1euq OCA], [https://pdbe.org/1euq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1euq RCSB], [https://www.ebi.ac.uk/pdbsum/1euq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1euq ProSAT]</span></td></tr> |
| | </table> |
| | == Function == |
| | [https://www.uniprot.org/uniprot/SYQ_ECOLI SYQ_ECOLI] |
| | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> |
| | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eu/1euq_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> |
| | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1euq ConSurf]. |
| | <div style="clear:both"></div> |
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| '''CRYSTAL STRUCTURE OF GLUTAMINYL-TRNA SYNTHETASE COMPLEXED WITH A TRNA-GLN MUTANT AND AN ACTIVE-SITE INHIBITOR'''
| | ==See Also== |
| | | *[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]] |
| | | *[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]] |
| ==Overview== | | __TOC__ |
| The position of the tertiary Levitt pair between nucleotides 15 and 48 in the transfer RNA core region suggests a key role in stabilizing the joining of the two helical domains, and in maintaining the relative orientations of the D and variable loops. E. coli tRNA(Gln) possesses the canonical Pu15-Py48 trans pairing at this position (G15-C48), while the tRNA(Cys) species from this organism instead features an unusual G15-G48 pair. To explore the structural context dependence of a G15-G48 Levitt pair, a number of tRNA(Gln) species containing G15-G48 were constructed and evaluated as substrates for glutaminyl and cysteinyl-tRNA synthetases. The glutaminylation efficiencies of these mutant tRNAs are reduced by two to tenfold compared with native tRNA(Gln), consistent with previous findings that the tertiary core of this tRNA plays a role in GlnRS recognition. Introduction of tRNA(Cys) identity nucleotides at the acceptor and anticodon ends of tRNA(Gln) produced a tRNA substrate which was efficiently aminoacylated by CysRS, even though the tertiary core region of this species contains the tRNA(Gln) G15-C48 pair. Surprisingly, introduction of G15-G48 into the non-cognate tRNA(Gln) tertiary core then significantly impairs CysRS recognition. By contrast, previous work has shown that CysRS aminoacylates tRNA(Cys) core regions containing G15-G48 with much better efficiency than those with G15-C48. Therefore, tertiary nucleotides surrounding the Levitt pair must significantly modulate the efficiency of aminoacylation by CysRS. To explore the detailed nature of the structural interdependence, crystal structures of two tRNA(Gln) mutants containing G15-G48 were determined bound to GlnRS. These structures show that the larger purine ring of G48 is accommodated by rotation into the syn position, with the N7 nitrogen serving as hydrogen bond acceptor from several groups of G15. The G15-G48 conformations differ significantly compared to that observed in the native tRNA(Cys) structure bound to EF-Tu, further implicating an important role for surrounding nucleotides in maintaining the integrity of the tertiary core and its consequent ability to present crucial recognition determinants to aminoacyl-tRNA synthetases.
| | </StructureSection> |
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| ==About this Structure==
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| 1EUQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The following page contains interesting information on the relation of 1EUQ with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb16_1.html Aminoacyl-tRNA Synthetases]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EUQ OCA].
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| ==Reference==
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| Influence of transfer RNA tertiary structure on aminoacylation efficiency by glutaminyl and cysteinyl-tRNA synthetases., Sherlin LD, Bullock TL, Newberry KJ, Lipman RS, Hou YM, Beijer B, Sproat BS, Perona JJ, J Mol Biol. 2000 Jun 2;299(2):431-46. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10860750 10860750]
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| [[Category: Aminoacyl-tRNA Synthetases]] | | [[Category: Aminoacyl-tRNA Synthetases]] |
| [[Category: Escherichia coli]] | | [[Category: Escherichia coli]] |
| [[Category: Glutamine--tRNA ligase]] | | [[Category: Large Structures]] |
| [[Category: Single protein]] | | [[Category: RCSB PDB Molecule of the Month]] |
| [[Category: Beijer, B.]] | | [[Category: Beijer B]] |
| [[Category: Bullock, T L.]] | | [[Category: Bullock TL]] |
| [[Category: Hou, Y M.]] | | [[Category: Hou Y-M]] |
| [[Category: Lipman, R S.A.]] | | [[Category: Lipman RSA]] |
| [[Category: Newberry, K J.]] | | [[Category: Newberry KJ]] |
| [[Category: Perona, J J.]] | | [[Category: Perona JJ]] |
| [[Category: Sherlin, L D.]] | | [[Category: Sherlin LD]] |
| [[Category: Sproat, B S.]] | | [[Category: Sproat BS]] |
| [[Category: E. coli]]
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| [[Category: Glutamine]]
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| [[Category: Rna/protein complex]]
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| [[Category: Trna synthetase]]
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| [[Category: Trnagln]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:32:19 2008''
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