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[[Image:1esg.gif|left|200px]]<br /><applet load="1esg" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1esg, resolution 1.90&Aring;" />
'''RESTRICTION ENDONUCLEASE BAMHI BOUND TO A NON-SPECIFIC DNA.'''<br />


==Overview==
==RESTRICTION ENDONUCLEASE BAMHI BOUND TO A NON-SPECIFIC DNA.==
The central problem faced by DNA binding proteins is how to select the correct DNA sequence from the sea of nonspecific sequences in a cell. The problem is particularly acute for bacterial restriction enzymes because cleavage at an incorrect DNA site could be lethal. To understand the basis of this selectivity, we report here the crystal structure of endonuclease BamHI bound to noncognate DNA. We show that, despite only a single base pair change in the recognition sequence, the enzyme adopts an open configuration that is on the pathway between free and specifically bound forms of the enzyme. Surprisingly, the DNA drops out of the binding cleft with a total loss of base-specific and backbone contacts. Taken together, the structure provides a remarkable snapshot of an enzyme poised for linear diffusion (rather than cleavage) along the DNA.
<StructureSection load='1esg' size='340' side='right'caption='[[1esg]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1esg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ESG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1esg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1esg OCA], [https://pdbe.org/1esg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1esg RCSB], [https://www.ebi.ac.uk/pdbsum/1esg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1esg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/T2BA_BACAM T2BA_BACAM] Recognizes the double-stranded sequence GGATCC and cleaves after G-1.


==About this Structure==
==See Also==
1ESG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESG OCA].
*[[BamHI|BamHI]]
 
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
==Reference==
__TOC__
Structure of BamHI bound to nonspecific DNA: a model for DNA sliding., Viadiu H, Aggarwal AK, Mol Cell. 2000 May;5(5):889-95. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10882125 10882125]
</StructureSection>
[[Category: Bacillus amyloliquefaciens]]
[[Category: Bacillus amyloliquefaciens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Type II site-specific deoxyribonuclease]]
[[Category: Aggarwal AK]]
[[Category: Aggarwal, A K.]]
[[Category: Viadiu H]]
[[Category: Viadiu, H.]]
[[Category: non-specific dna-protein complex.]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:31:01 2008''

Latest revision as of 10:06, 7 February 2024

RESTRICTION ENDONUCLEASE BAMHI BOUND TO A NON-SPECIFIC DNA.RESTRICTION ENDONUCLEASE BAMHI BOUND TO A NON-SPECIFIC DNA.

Structural highlights

1esg is a 4 chain structure with sequence from Bacillus amyloliquefaciens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.9Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

T2BA_BACAM Recognizes the double-stranded sequence GGATCC and cleaves after G-1.

See Also

1esg, resolution 1.90Å

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